Abstract
Inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC 3.6.1.1) was purified from pig scapula cartilage by fractionation with N-cetylpyridinium chloride and (NH4)2SO4, followed by ion-exchange and gel-filtration chromatography. Enzyme preparations of high purity were obtained, with specific activities (100-400 units/mg) higher than those previously described for mammalian pyrophosphatases. The enzyme activity could be separated into several subfractions on ion-exchange columns.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alcock N. W., Shils M. E. Association of inorganic pyrophosphatase activity with normal calcification of rat costal cartilage in vivo. Biochem J. 1969 May;112(4):505–510. doi: 10.1042/bj1120505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buruiana L. M., Hadarag E. Isozymes of the pyrophosphatase from the brain. Experientia. 1968 Jul 15;24(7):664–665. doi: 10.1007/BF02138299. [DOI] [PubMed] [Google Scholar]
- Chen M., McCarry J., Chan M. M., Riggins R. S., Rucker R. B. Chick aorta pyrophosphatase. Proc Soc Exp Biol Med. 1973 May;143(1):44–49. doi: 10.3181/00379727-143-37249. [DOI] [PubMed] [Google Scholar]
- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- Eifler R., Hahn V., Hermann I. On the origin of "isozymes" of inorganic pyrophosphatase obtained from baker's yeast. Acta Biol Med Ger. 1972;28(4):707–709. [PubMed] [Google Scholar]
- Felix R., Fleisch H. Properties of inorganic pyrophosphatase of pig scapula cartilage. Biochem J. 1975 Apr;147(1):111–118. doi: 10.1042/bj1470111. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Irie M., Yabuta A., Kimura K., Shindo Y., Tomita K. Distribution and properties of alkaline pyrophosphatases of rat liver. J Biochem. 1970 Jan;67(1):47–58. doi: 10.1093/oxfordjournals.jbchem.a129233. [DOI] [PubMed] [Google Scholar]
- KUNITZ M. Crystalline inorganic pyrophosphatase isolated from baker's yeast. J Gen Physiol. 1952 Jan;35(3):423–450. doi: 10.1085/jgp.35.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kesselring K., Siebert G. Eigenschaften einer löslichen anorganischen Pyrophosphatase aus Rattenleber-Zellkernen. Hoppe Seylers Z Physiol Chem. 1967 May;348(5):585–598. [PubMed] [Google Scholar]
- Kuettner K. E., Guenther H. L., Ray R. D., Schumacher G. F. Lysozyme in preosseous cartilage. Calcif Tissue Res. 1968 Mar 27;1(4):298–305. doi: 10.1007/BF02008101. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- NORDLIE R. C., LARDY H. A. Sub-cellular distribution of ratliver inorganic pyrophosphatase activity. Biochim Biophys Acta. 1961 Jun 10;50:189–191. doi: 10.1016/0006-3002(61)91083-6. [DOI] [PubMed] [Google Scholar]
- PYNES G. D., YOUNATHAN E. S. THE SUBCELLULAR DISTRIBUTION OF INORGANIC PYROPHOSPHATASE IN PIGEON PANCREAS. Biochim Biophys Acta. 1964 Oct 23;92:150–151. doi: 10.1016/0926-6569(64)90279-2. [DOI] [PubMed] [Google Scholar]
- Pynes G. D., Younathan E. S. Purification and some properties of inorganic pyrophosphatase from human erythrocytes. J Biol Chem. 1967 May 10;242(9):2119–2123. [PubMed] [Google Scholar]
- Schick L., Butler L. G. Inorganic pyrophosphatase of rat liver mitochondria. Correlation of latency with catalytic properties and intramitochondrial location. J Cell Biol. 1969 Jul;42(1):235–240. doi: 10.1083/jcb.42.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tono H., Kornberg A. Biochemical studies of bacterial sporulation. 3. Inorganic pyrophosphatase of vegetative cells and spores of Bacillus subtilis. J Biol Chem. 1967 May 25;242(10):2375–2382. [PubMed] [Google Scholar]
- Wöltgens J., Ahsmann W. Determination of orthophosphate in the presence of inorganic pyrophosphate in the assay of inorganic pyrophosphatase activity. Anal Biochem. 1970 Jun;35(2):526–529. doi: 10.1016/0003-2697(70)90217-4. [DOI] [PubMed] [Google Scholar]