Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1975 May;147(2):193–197. doi: 10.1042/bj1470193

Ribonucleic acid synthesis in isolated rat liver nuclei under conditions of ribonucleic acid processing and transport.

D J McNamara, J Racevskis, D E Schumm, T E Webb
PMCID: PMC1165430  PMID: 1180886

Abstract

A cell-free system is described which permits a significant and prolonged synthesis of RNA in isolated rat liver nuclei, under conditions previously demonstrated to support normal nuclear processing and transport of both rRNA and mRNA. The system contains cytosol but not (NH4)2SO4 or other non-physiological components. Evidence is presented for cytosol factors which stimulate ribosomal, and to a lesser degree, non-ribosomal RNA synthesis.

Full text

PDF
193

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banks S. P., Gilbert B. E., Johnson T. C. Stimulation of RNA synthesis in isolated mouse brain nuclei by a brain cytosol factor. Life Sci. 1974 Jan 16;14(2):303–310. doi: 10.1016/0024-3205(74)90060-5. [DOI] [PubMed] [Google Scholar]
  2. Chang C. S., Goldwasser E. On the mechanism of erythropoietin-induced differentiation. XII. A cytoplasmic protein mediating induced nuclear RNA synthesis. Dev Biol. 1973 Oct;34(2):246–254. doi: 10.1016/0012-1606(73)90353-9. [DOI] [PubMed] [Google Scholar]
  3. DeLarco J., Guroff G. The binding of RNA to various celluloses. Biochem Biophys Res Commun. 1973 Jan 23;50(2):486–492. doi: 10.1016/0006-291x(73)90866-8. [DOI] [PubMed] [Google Scholar]
  4. Froehner S. C., Bonner J. Ascites tumor ribonucleic acid polymerases. Isolation, purification, and factor stimulation. Biochemistry. 1973 Jul 31;12(16):3064–3071. doi: 10.1021/bi00740a019. [DOI] [PubMed] [Google Scholar]
  5. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  6. Grummt I., Lindigkeit R. Pre-ribosomal RNA synthesis in isolated rat-liver nucleoli. Eur J Biochem. 1973 Jul 2;36(1):244–249. doi: 10.1111/j.1432-1033.1973.tb02906.x. [DOI] [PubMed] [Google Scholar]
  7. Higashinakagawa T., Onishi T., Muramatsu M. A factor stimulating the transcription by nucleolar RNA polymerase in the nucleolus of rat liver. Biochem Biophys Res Commun. 1972 Aug 21;48(4):937–944. doi: 10.1016/0006-291x(72)90699-7. [DOI] [PubMed] [Google Scholar]
  8. Novello F., Stirpe F. Simultaneous assay of RNA polymerase I and II in nuclei isolated from resting and growing rat liver with the use of alpha-amanitin. FEBS Lett. 1970 May 11;8(1):57–60. doi: 10.1016/0014-5793(70)80225-3. [DOI] [PubMed] [Google Scholar]
  9. Ono T., Kawamura M., Hyodo M., Wakabayashi K. Difference of RNA population between normal liver and hepatoma AH-130. Gan. 1971 Feb;62(1):31–40. [PubMed] [Google Scholar]
  10. Schumm D. E., Morris H. P., Webb T. E. Cytosol-modulated transport of messenger RNA from isolated nuclei. Cancer Res. 1973 Aug;33(8):1821–1828. [PubMed] [Google Scholar]
  11. Schumm D. E., Webb T. E. Modified messenger ribonucleic acid release from isolated hepatic nuclei after inhibition of polyadenylate formation. Biochem J. 1974 Apr;139(1):191–196. doi: 10.1042/bj1390191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schumm D. E., Webb T. E. The in vivo equivalence of a cell-free system for RNA processing and transport. Biochem Biophys Res Commun. 1974 May 20;58(2):354–360. doi: 10.1016/0006-291x(74)90372-6. [DOI] [PubMed] [Google Scholar]
  13. Schumm D. E., Webb T. E. Transport of informosomes from isolated nuclei of regenerating rat liver. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1259–1265. doi: 10.1016/0006-291x(72)90847-9. [DOI] [PubMed] [Google Scholar]
  14. Seifart K. H., Juhasz P. P., Benecke B. J. A protein factor from rat-liver tissue enhancing the transcription of native templates by homologous RNA polymerase B. Eur J Biochem. 1973 Feb 15;33(1):181–191. doi: 10.1111/j.1432-1033.1973.tb02668.x. [DOI] [PubMed] [Google Scholar]
  15. Soll D. R., Sussman M. Transcription in isolated nuclei of the sline mold Dictyostelium discoideum. Biochim Biophys Acta. 1973 Sep 7;319(3):312–322. doi: 10.1016/0005-2787(73)90171-8. [DOI] [PubMed] [Google Scholar]
  16. Stein H., Hausen P. A factor from calf thymus stimulating DNA-dependent RNA polymerase isolated from this tissue. Eur J Biochem. 1970 Jun;14(2):270–277. doi: 10.1111/j.1432-1033.1970.tb00286.x. [DOI] [PubMed] [Google Scholar]
  17. Summers W. C. A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate. Anal Biochem. 1970 Feb;33(2):459–463. doi: 10.1016/0003-2697(70)90316-7. [DOI] [PubMed] [Google Scholar]
  18. Thompson L. R., McCarthy B. J. Stimulation of nuclear DNA and RNA synthesis by cytoplasmic extracts in vitro. Biochem Biophys Res Commun. 1968 Jan 25;30(2):166–172. doi: 10.1016/0006-291x(68)90465-8. [DOI] [PubMed] [Google Scholar]
  19. Weinmann R., Roeder R. G. Role of DNA-dependent RNA polymerase 3 in the transcription of the tRNA and 5S RNA genes. Proc Natl Acad Sci U S A. 1974 May;71(5):1790–1794. doi: 10.1073/pnas.71.5.1790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Widnell C. C., Tata J. R. A procedure for the isolation of enzymically active rat-liver nuclei. Biochem J. 1964 Aug;92(2):313–317. doi: 10.1042/bj0920313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Younger L. R., Gelboin H. V. The electrophoretic distribution of RNA synthesized in vitro by isolated rat liver nuclei. Biochim Biophys Acta. 1970 Mar 19;204(1):168–174. doi: 10.1016/0005-2787(70)90499-5. [DOI] [PubMed] [Google Scholar]
  22. Yu L. C., Racevskis J., Webb T. E. Regulated transport of ribosomal subunits from regenerating rat liver nuclei in a cell-free system. Cancer Res. 1972 Nov;32(11):2314–2321. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES