Abstract
Free uroporphyrin is decarboxylated by heating in the air to 150--200 degrees C. Complete decarboxylation occurs after heating to 200 degrees C for 5 min, with a yield of 66--75%. Heptacarboxylic porphyrin can be prepared with a yield of 40% by heating uroporphyrin to 150 degrees C for 60 min, and hexacarboxylic porphyrin with a yield of about 20% by heating to 150 degrees C for 2h.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHU T. C., CHU E. J. Hepta-, hexa-, and pentacarboxylic porphyrins of porphyria cutanea tarda. II. Preparation of the porphyrins by stepwise decarboxylation ofuroporphyrins. J Biol Chem. 1959 Oct;234:2747–2750. [PubMed] [Google Scholar]
- Doss M. Analytical and preparative thin-layer chromatography of porphyrin methyl esters. Z Klin Chem Klin Biochem. 1970 May;8(3):197–207. doi: 10.1515/cclm.1970.8.3.197. [DOI] [PubMed] [Google Scholar]
- EDMONDSON P. R., SCHWARTZ S. Studies of the uroporphyrins. III. An improved method for the decarboxylation of uroporphyrin. J Biol Chem. 1953 Dec;205(2):605–609. [PubMed] [Google Scholar]
- RIMINGTON C., SVEINSSON S. L. The spectrophotometric determination of uroporphyrin. Scand J Clin Lab Invest. 1950;2(3):209–216. doi: 10.3109/00365515009049872. [DOI] [PubMed] [Google Scholar]
- Rimington C. Spectral-absorption coefficients of some porphyrins in the Soret-band region. Biochem J. 1960 Jun;75(3):620–623. doi: 10.1042/bj0750620. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rimington C., With T. K. Sub-uroporphyrins and their occurrence. A preliminary communication. Enzyme. 1974;17(1):17–28. doi: 10.1159/000459302. [DOI] [PubMed] [Google Scholar]
- WITH T. K. Preparation of crystalline porphyrin esters from bovine porphyria urine. Biochem J. 1958 Apr;68(4):717–720. doi: 10.1042/bj0680717. [DOI] [PMC free article] [PubMed] [Google Scholar]
- With T. K. Preparation of crystalline porphyrin esters and free porphyrins from natural products. Dan Med Bull. 1969 Oct;16(9):268–279. [PubMed] [Google Scholar]
- With T. K. Simplified decarboxylation of uroporphyrins. Enzyme. 1974;17(1):76–80. doi: 10.1159/000459310. [DOI] [PubMed] [Google Scholar]
- With T. K. Thin-layer chromatography of free and esterified porphyrins on talc. J Chromatogr. 1969 Jul;42(3):389–395. doi: 10.1016/s0021-9673(01)80639-7. [DOI] [PubMed] [Google Scholar]