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. 1975 Jun;148(3):551–555. doi: 10.1042/bj1480551

Desaturation of stearic acid by liver and adipose tissue from obese-hyperglycaemic mice (ob/ob).

M Enser
PMCID: PMC1165576  PMID: 992

Abstract

Stearic acid desaturase activity was assayed in preparations from perigenital adipose tissue and liver from lean and genetically obese female mice (ob/ob). The total activity in the perigenital adipose tissue from obese mice was threefold greater than in the tissue from lean mice, but per g of adipose tissue the activity was twofold greater in tissue from lean mice. In liver, the activity in obese mice was elevated at 8 weeks of age, remained elevated up to 24 weeks and then decreased by half at 48 weeks, but at all ages was higher than that in lean mice. The decrease in desaturase activity of liver from obese mice at 48 weeks corresponded to a change in the fatty acid composition of liver lipids toward that found in lean mice. Whereas in adipose tissue much of the increased enzyme activity may be due to tissue hyperplasia, in liver it is mainly an increased activity per cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bray G. A., York D. A. Genetically transmitted obesity in rodents. Physiol Rev. 1971 Jul;51(3):598–646. doi: 10.1152/physrev.1971.51.3.598. [DOI] [PubMed] [Google Scholar]
  3. Bulfield G. Genetic control of metabolism: enzyme studies of the obese and adipose mutants in the mouse. Genet Res. 1972 Aug;20(1):51–64. doi: 10.1017/s0016672300013586. [DOI] [PubMed] [Google Scholar]
  4. CHRISTOPHE J., JEANRENAUD B., MAYER J., RENOLD A. E. Metabolism in vitro of adipose tissue in obese-hyperglycemic and goldthioglucose-treated mice. II. Metabolism of pyruvate and acetate. J Biol Chem. 1961 Mar;236:648–652. [PubMed] [Google Scholar]
  5. Chang H. C., Seidman I., Teebor G., Lane M. D. Liver acetyl CoA carboxylase and fatty acid synthetase: relative activities in the normal state and in hereditary obesity. Biochem Biophys Res Commun. 1967 Sep 7;28(5):682–686. doi: 10.1016/0006-291x(67)90369-5. [DOI] [PubMed] [Google Scholar]
  6. DOLE V. P., MEINERTZ H. Microdetermination of long-chain fatty acids in plasma and tissues. J Biol Chem. 1960 Sep;235:2595–2599. [PubMed] [Google Scholar]
  7. Enser M. Clearing-factor lipase in obese hyperglycaemic mice (ob-ob). Biochem J. 1972 Sep;129(2):447–453. doi: 10.1042/bj1290447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  9. GELLHORN A., BENJAMIN W. THE INTRACELLULAR LOCALIZATION OF AN ENZYMATIC DEFECT OF LIPID METABOLISM IN DIABETIC RATS. Biochim Biophys Acta. 1964 Apr 20;84:167–175. doi: 10.1016/0926-6542(64)90073-3. [DOI] [PubMed] [Google Scholar]
  10. GETZ G. S., BARTLEY W. The intracellular distribution of fatty acids in rat liver. The fatty acids of intracellular compartments. Biochem J. 1961 Feb;78:307–312. doi: 10.1042/bj0780307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HOLLIFIELD G., PARSON W., AYERS C. R. In vitro synthesis of lipids from C-14 acetate by adipose tissue from four types of obese mice. Am J Physiol. 1960 Jan;198:37–38. doi: 10.1152/ajplegacy.1960.198.1.37. [DOI] [PubMed] [Google Scholar]
  12. Haessler H. A., Crawford J. D. Alterations in the fatty acid composition of depot fat associated with obesity. Ann N Y Acad Sci. 1965 Oct 8;131(1):476–484. doi: 10.1111/j.1749-6632.1965.tb34813.x. [DOI] [PubMed] [Google Scholar]
  13. Herberg L., Major E., Hennigs U., Grüneklee D., Freytag G., Gries F. A. Differences in the development of the obese-hyperglycemic syndrome in obob and NZO mice. Diabetologia. 1970 Jun;6(3):292–299. doi: 10.1007/BF01212241. [DOI] [PubMed] [Google Scholar]
  14. Inkpen C. A., Harris R. A., Quackenbush F. W. Differential responses to fasting and subsequent feeding by microsomal systems of rat liver: 6- and 9-desaturation of fatty acids. J Lipid Res. 1969 May;10(3):277–282. [PubMed] [Google Scholar]
  15. Jansen G. R., Zanetti M. E., Hutchison C. F. Studies on lipogenesis in vivo: Fatty acid and cholesterol synthesis in hyperglycaemic-obese mice. Biochem J. 1967 Mar;102(3):870–877. doi: 10.1042/bj1020870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnson P. R., Hirsch J. Cellularity of adipose depots in six strains of genetically obese mice. J Lipid Res. 1972 Jan;13(1):2–11. [PubMed] [Google Scholar]
  17. KORNACKER M. S., LOWENSTEIN J. M. CITRATE CLEAVAGE ENZYME IN LIVERS OF OBESE AND NONOBESE MICE. Science. 1964 May 22;144(3621):1027–1028. doi: 10.1126/science.144.3621.1027. [DOI] [PubMed] [Google Scholar]
  18. Kaplan A. A simple radioactive assay for triglyceride lipase. Anal Biochem. 1970 Feb;33(2):218–225. doi: 10.1016/0003-2697(70)90289-7. [DOI] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. Lemonnier D., Winand J., Furnelle J., Christophe J. Effect of a high-fat diet on obese-hyperglycaemic and non-obese Bar Harbor mice. Diabetologia. 1971 Oct;7(5):328–333. doi: 10.1007/BF01219466. [DOI] [PubMed] [Google Scholar]
  21. MARSH J. B., JAMES A. T. The conversion of stearic to oleic acid by liver and yeast preparations. Biochim Biophys Acta. 1962 Jul 2;60:320–328. doi: 10.1016/0006-3002(62)90407-9. [DOI] [PubMed] [Google Scholar]
  22. Stauffacher W., Lambert A. E., Vecchio D., Renold A. E. Measurements of insulin activities in pancreas and serum of mice with spontaneous ("Obese" and "New Zealand Obese") and induced (Goldthioglucose) obesity and hyperglycemia, with considerations on the pathogenesis of the spontaneous syndrome. Diabetologia. 1967 Apr;3(2):230–237. doi: 10.1007/BF01222200. [DOI] [PubMed] [Google Scholar]
  23. Uchiyama M., Nakagawa M., Okui S. Effect of free unsaturated fatty acids on fatty acid desaturation by liver preparations. J Biochem. 1967 Jul;62(1):1–6. doi: 10.1093/oxfordjournals.jbchem.a128621. [DOI] [PubMed] [Google Scholar]
  24. Winand J., Furnelle J., Christophe J. Le métabolisme lipidique du foie chez la souris normale et la souris obèse-hyperglycémique. Biochim Biophys Acta. 1968 Mar 4;152(2):280–292. [PubMed] [Google Scholar]
  25. Winand J., Furnelle J., Wodon C., Hebbelinck M., Christophe J. 7-Day time study of lipid metabolism in normal and obese-hyperglycemic Bar Harbor mice. Qualitative and quantitative aspects. Biochimie. 1973;55(1):63–73. doi: 10.1016/s0300-9084(73)80238-x. [DOI] [PubMed] [Google Scholar]

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