Abstract
Purified satellite DNA from melon (Cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. Two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite DNA, and one with 6000 times greater complexity. Both components renatured very accurately, indicating a minimum of sequence divergence. Centrifugation of the purified satellite DNA in Ag+/Cs2SO4 gradients resolved two major and several minor fractions. The two major fractions were only slightly enriched for fast- or slow-renaturing sequences.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arrighi F. E., Mandel M., Bergendahl J., Hsu T. C. Buoyant densities of DNA of mammals. Biochem Genet. 1970 Jun;4(3):367–376. doi: 10.1007/BF00485753. [DOI] [PubMed] [Google Scholar]
- Chilton M. D. Theoretical explanation of mouse satellite DNA renaturation kinetics. Nat New Biol. 1973 Nov 7;246(149):16–17. doi: 10.1038/newbio246016a0. [DOI] [PubMed] [Google Scholar]
- Corneo G., Ginelli E., Soave C., Bernardi G. Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids. Biochemistry. 1968 Dec;7(12):4373–4379. doi: 10.1021/bi00852a033. [DOI] [PubMed] [Google Scholar]
- Freifelder D. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. J Mol Biol. 1970 Dec 28;54(3):567–577. doi: 10.1016/0022-2836(70)90127-0. [DOI] [PubMed] [Google Scholar]
- Gall J. G., Cohen E. H., Polan M. L. Reptitive DNA sequences in drosophila. Chromosoma. 1971;33(3):319–344. doi: 10.1007/BF00284948. [DOI] [PubMed] [Google Scholar]
- Gillis M., De Ley J., De Cleene M. The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem. 1970 Jan;12(1):143–153. doi: 10.1111/j.1432-1033.1970.tb00831.x. [DOI] [PubMed] [Google Scholar]
- Ingle J., Pearson G. G., Sinclair J. Species distribution and properties of nuclear satellite DNA in higher plants. Nat New Biol. 1973 Apr 18;242(120):193–197. doi: 10.1038/newbio242193a0. [DOI] [PubMed] [Google Scholar]
- Kolodner R., Tewari K. K. Physicochemical characterization of mitochondrial DNA from pea leaves. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1830–1834. doi: 10.1073/pnas.69.7.1830. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
- Scott N. S., Ingle J. The genes for cytoplasmic ribosomal ribonucleic Acid in higher plants. Plant Physiol. 1973 Apr;51(4):677–684. doi: 10.1104/pp.51.4.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sutton W. D., McCallum M. Mismatching and the reassociation rate of mouse satellite DNA. Nat New Biol. 1971 Jul 21;232(29):83–85. doi: 10.1038/newbio232083a0. [DOI] [PubMed] [Google Scholar]
- Wells R., Birnstiel M. Kinetic complexity of chloroplastal deoxyribonucleic acid and mitochondrial deoxyribonucleic acid from higher plants. Biochem J. 1969 May;112(5):777–786. doi: 10.1042/bj1120777. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wells R., Ingle J. The constancy of the buoyant density of chloroplast and mitochondrial deoxyribonucleic acids in a range of higher plants. Plant Physiol. 1970 Jul;46(1):178–179. doi: 10.1104/pp.46.1.178. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]
