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. 1975 Jul;149(1):297–300. doi: 10.1042/bj1490297

A sensitive radiochemical assay for angiotensin-converting enzyme (kinase II).

A T Chiu, J W Ryan, U S Ryan
PMCID: PMC1165618  PMID: 172070

Abstract

[[125I]Tyr8]Bradykinin is degraded by angiotensin-converting enzyme to [125I]Tyr-Arg. The reaction product can be separated completely and recovered nearly quantitatively from unchanged substrate by cation-exchange chromatography. Thus it is possible to use [[125I]Tyr8]bradykinin at high specific radioactivity (about 400Ci/mmol) to measure the small quantities of angiotensin-converting enzyme encountered in small-scale cultures of pulmonary endothelial cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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