Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1975 Jul;150(1):133–135. doi: 10.1042/bj1500133

The early enhancement of rat liver deoxyribonucleic acid-dependent ribonucleic acid polymerase II activity by tri-iodothyronine.

S Jothy, J L Bilodeau, H Champsaur, H Simpkins
PMCID: PMC1165713  PMID: 1201005

Abstract

It is shown that tri-iodothyronine injected intravenously into thyroidectomized rats induces an early and transient activation of rat liver RNA polymerase II which could be demonstrated to occur 40-80 min after hormonal treatment. There was a simultaneous increase in the concentration of acidic proteins bound to chromatin.

Full text

PDF
133

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baulieu E. E., Wira C. R., Milgrom E., Raynaud-Jammet C. Ribonucleic acid synthesis and oestradiol action in the uterus. Acta Endocrinol Suppl (Copenh) 1972;168:396–419. doi: 10.1530/acta.0.071s396. [DOI] [PubMed] [Google Scholar]
  3. Blobel G., Potter V. R. Nuclei from rat liver: isolation method that combines purity with high yield. Science. 1966 Dec 30;154(3757):1662–1665. doi: 10.1126/science.154.3757.1662. [DOI] [PubMed] [Google Scholar]
  4. Chambon P., Ramuz M., Mandel P., Doly J. The influence of ionic strength and a polyanion on transcription in vitro. I. Stimulation of the aggregate RNA polymerase from rat liver nuclei. Biochim Biophys Acta. 1968 May 21;157(3):505–519. [PubMed] [Google Scholar]
  5. DINGMAN C. W., SPORN M. B. STUDIES ON CHROMATIN. I. ISOLATION AND CHARACTERIZATION OF NUCLEAR COMPLEXES OF DEOXYRIBONUCLEIC ACID, RIBONUCLEIC ACID, AND PROTEIN FROM EMBRYONIC AND ADULT TISSUES OF THE CHICKEN. J Biol Chem. 1964 Oct;239:3483–3492. [PubMed] [Google Scholar]
  6. Glasser S. R., Chytil F., Spelsberg T. C. Early effects of oestradiol-17 on the chromatin and activity of the deoxyribonucleic acid-dependent ribonucleic acid polymerases (I and II) of the rat uterus. Biochem J. 1972 Dec;130(4):947–957. doi: 10.1042/bj1300947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kamiyama M., Wang T. Y. Activated transcription from rat liver chromatin by non-histone proteins. Biochim Biophys Acta. 1971 Jan 28;228(2):563–576. doi: 10.1016/0005-2787(71)90062-1. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Munro H. N., Fleck A. Recent developments in the measurement of nucleic acids in biological materials. A supplementary review. Analyst. 1966 Feb;91(79):78–88. doi: 10.1039/an9669100078. [DOI] [PubMed] [Google Scholar]
  10. Oppenheimer J. H., Schwartz H. L., Koerner D., Surks M. I. Limited binding capacity sites for L-triiodothyronine in rat liver nuclei. Nuclear-cytoplasmic interrelation, binding constants, and cross-reactivity with L-thyroxine. J Clin Invest. 1974 Mar;53(3):768–777. doi: 10.1172/JCI107615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smuckler E. A., Tata J. R. Changes in hepatic nuclear DNA-dependent RNA polymerase caused by growth hormone and triiodothyronine. Nature. 1971 Nov 5;234(5323):37–39. doi: 10.1038/234037a0. [DOI] [PubMed] [Google Scholar]
  12. Spelsberg T. C., Steggles A. W., O'Malley B. W. Progesterone-binding components of chick oviduct. 3. Chromatin acceptor sites. J Biol Chem. 1971 Jul 10;246(13):4188–4197. [PubMed] [Google Scholar]
  13. Zerwekh J. E., Haussler M. R., Lindell T. J. Rapid enhancement of chick intestinal DNA-dependent RNA polymerase II activity by 1 alpha, 25-dihydroxyvitamin D3, in vivo. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2337–2341. doi: 10.1073/pnas.71.6.2337. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES