Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1973 Sep;135(1):19–30. doi: 10.1042/bj1350019

The structure of a β-(1→3)-d-glucan from yeast cell walls

David J Manners 1, Alan J Masson 1, James C Patterson 1
PMCID: PMC1165784  PMID: 4359920

Abstract

Yeast glucan as normally prepared by various treatments of yeast (Saccharomyces cerevisiae) cell walls to remove mannan and glycogen is still heterogeneous. The major component (about 85%) is a branched β-(1→3)-glucan of high molecular weight (about 240000) containing 3% of β-(1→6)-glucosidic interchain linkages. The minor component is a branched β-(1→6)-glucan. A comparison of our results with those of other workers suggests that different glucan preparations may differ in the degree of heterogeneity and that the major β-(1→3)-glucan component may vary considerably in degree of branching.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bacon J. S., Farmer V. C., Jones D., Taylor I. F. The glucan components of the cell wall of baker's yeast (Saccharomyces cerevisiae) considered in relation to its ultrastructure. Biochem J. 1969 Sep;114(3):557–567. doi: 10.1042/bj1140557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bacon J. S., Gordon A. H., Jones D., Taylor I. F., Webley D. M. The separation of beta-glucanases produced by Cytophaga johnsonii and their role in the lysis of yeast cell walls. Biochem J. 1970 Nov;120(1):67–78. doi: 10.1042/bj1200067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hopkins R. P., Young L. Biochemical studies of toxic agents. Metabolic ring-fission of cis- and trans-acenaphthene-1,2-diol. Biochem J. 1966 Jan;98(1):19–24. doi: 10.1042/bj0980019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kidby D. K., Davies R. Invertase and disulphide bridges in the yeast wall. J Gen Microbiol. 1970 Jun;61(3):327–333. doi: 10.1099/00221287-61-3-327. [DOI] [PubMed] [Google Scholar]
  5. Lampen J. O. External enzymes of yeast: their nature and formation. Antonie Van Leeuwenhoek. 1968;34(1):1–18. doi: 10.1007/BF02046409. [DOI] [PubMed] [Google Scholar]
  6. MANNERS D. J. The molecular structure of glycogens. Adv Carbohydr Chem. 1957;12:261–298. doi: 10.1016/s0096-5332(08)60210-6. [DOI] [PubMed] [Google Scholar]
  7. Manners D. J., Masson A. J., Patterson J. C., Björndal H., Lindberg B. The structure of a beta-(1--6)-D-glucan from yeast cell walls. Biochem J. 1973 Sep;135(1):31–36. doi: 10.1042/bj1350031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Manners D. J., Masson A. J. The structures of two glucans from yeast-cell walls. FEBS Lett. 1969 Jul;4(2):122–124. doi: 10.1016/0014-5793(69)80211-5. [DOI] [PubMed] [Google Scholar]
  9. Manners D. J., Wilson G. Studies on beta-glucanases. Some properties of a bacterial endo-beta-(1 leads to 3)-glucanase system. Biochem J. 1973 Sep;135(1):11–18. doi: 10.1042/bj1350011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PHAFF H. J. CELL WALL OF YEASTS. Annu Rev Microbiol. 1963;17:15–30. doi: 10.1146/annurev.mi.17.100163.000311. [DOI] [PubMed] [Google Scholar]
  11. REESE E. T., PARRISH F. W., MANDELS M. Beta-d-1, 6-Glucanases in fungi. Can J Microbiol. 1962 Jun;8:327–334. doi: 10.1139/m62-045. [DOI] [PubMed] [Google Scholar]
  12. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES