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. 1973 Dec;135(4):631–637. doi: 10.1042/bj1350631

The molecular-weight distribution of glycosaminoglycans

John J Hopwood 1,*, H Clem Robinson 1
PMCID: PMC1165877  PMID: 4273184

Abstract

1. A rapid and sensitive method for the accurate estimation of the molecular-weight distribution of keratan sulphate and chondroitin sulphate isolated from adult bovine nasal septum and intervertebral disc is described. The method utilizes gel chromatography of reductively labelled glycosaminoglycan and end-group estimation of number-average molecular weight for each fraction across the peak of eluted glycosaminoglycan. 2. Chain-length distribution data obtained by this procedure are used to evaluate mechanisms of chondroitin sulphate biosynthesis.

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Selected References

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  1. ANTONOPOULOS C. A., BORELIUS E., GARDELL S., HAMNSTROM B., SCOTT J. E. The precipitation of polyanions by long-chain aliphatic ammonium compounds. IV. Elution in salt solutions of mucopolysaccharide-quaternary ammonium complexes adsorbed on a support. Biochim Biophys Acta. 1961 Dec 9;54:213–226. doi: 10.1016/0006-3002(61)90360-2. [DOI] [PubMed] [Google Scholar]
  2. Anseth A., Antonopoulos C. A., Bjelle A., Fransson L. A. Fractionation and quantitative determination of keratan sulfate using cetylpyridinium chloride and ECTEOLA-cellulose. Biochim Biophys Acta. 1970 Sep 22;215(3):522–526. doi: 10.1016/0304-4165(70)90103-0. [DOI] [PubMed] [Google Scholar]
  3. BLUMBERG B. S., OGSTON A. G. Further evidence on the protein complexes of some hyaluronic acids. Biochem J. 1958 Jan;68(1):183–188. doi: 10.1042/bj0680183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BLUMBERG B. S., OGSTON A. G. The effects of proteolytic enzymes on the hyaluronic acid complex of ox synovial fluid. Biochem J. 1957 Jun;66(2):342–346. doi: 10.1042/bj0660342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Constantopoulos G., Dekaban A. S., Carroll W. R. Determination of molecular weight distribution of acid mucopolysaccharides by sephadex gel filtration. Anal Biochem. 1969 Oct 1;31(1):59–70. doi: 10.1016/0003-2697(69)90241-3. [DOI] [PubMed] [Google Scholar]
  6. Hjertquist S. O., Lemperg R. Identification and concentration of the glycosaminoglycans of human articular cartilage in relation to age and osteoarthritis. Calcif Tissue Res. 1972;10(3):223–237. doi: 10.1007/BF02012552. [DOI] [PubMed] [Google Scholar]
  7. Hjertquist S. O., Wasteson A. The molecular weight of chondroitin sulphate from human articular cartilage. Effect of age and of osteoarthritis. Calcif Tissue Res. 1972;10(1):31–37. doi: 10.1007/BF02012533. [DOI] [PubMed] [Google Scholar]
  8. Hoffman P., Mashburn T. A., Jr, Meyer K. Proteinpolysaccharide of bovine cartilage. II. The relation of keratan sulfate and chondroitin sulfate. J Biol Chem. 1967 Sep 10;242(17):3805–3809. [PubMed] [Google Scholar]
  9. Laurent T. C., Granath K. A. Fractionation of dextran and Ficoll by chromatography on Sephadex G-200. Biochim Biophys Acta. 1967 Mar 22;136(2):191–198. doi: 10.1016/0304-4165(67)90063-3. [DOI] [PubMed] [Google Scholar]
  10. Luscombe M., Phelps C. F. Action of degradative enzymes on the light fraction of bovine septa protein polysaccharide. Biochem J. 1967 Apr;103(1):103–109. doi: 10.1042/bj1030103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marler E., Davidson E. A. Structure of a polysaccharide protein complex. Proc Natl Acad Sci U S A. 1965 Aug;54(2):648–656. doi: 10.1073/pnas.54.2.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mason R. M., Wusteman F. S. The glycosaminoglycans of human tracheobronchial cartilage. Biochem J. 1970 Dec;120(4):777–785. doi: 10.1042/bj1200777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mathews M. B. Comparative biochemistry of chondroitin sulphate-proteins of cartilage and notochord. Biochem J. 1971 Nov;125(1):37–46. doi: 10.1042/bj1250037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Robinson H. C., Dorfman A. The sulfation of chondroitin sulfate in embryonic chick cartilage epiphyses. J Biol Chem. 1969 Jan 25;244(2):348–352. [PubMed] [Google Scholar]
  15. Robinson H. C., Hopwood J. J. The alkaline cleavage and borohydride reduction of cartilage proteoglycan. Biochem J. 1973 Jul;133(3):457–470. doi: 10.1042/bj1330457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. TREVELYAN W. E., HARRISON J. S. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J. 1952 Jan;50(3):298–303. doi: 10.1042/bj0500298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Telser A., Robinson H. C., Dorfman A. The biosynthesis of chondroitin-sulfate protein complex. Proc Natl Acad Sci U S A. 1965 Sep;54(3):912–919. doi: 10.1073/pnas.54.3.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wasteson A. A method for the determination of molecular weight dispersion in chondroitin sulphate on a microgram level. Biochim Biophys Acta. 1969 Feb 18;177(1):152–154. doi: 10.1016/0304-4165(69)90076-2. [DOI] [PubMed] [Google Scholar]
  19. Wasteson A. A method for the determination of the molecular weight and molecular-weight distribution of chondroitin sulphate. J Chromatogr. 1971 Jul 8;59(1):87–97. doi: 10.1016/s0021-9673(01)80009-1. [DOI] [PubMed] [Google Scholar]

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