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. 1973 Dec;135(4):901–903. doi: 10.1042/bj1350901

The amino acid sequence around the reactive cysteine residue in human plasma Factor XIII (Short Communication)

J John Holbrook 1, Rodney D Cooke 1, I Barry Kingston 1
PMCID: PMC1165912  PMID: 4778285

Abstract

1. Activated human plasma factor XIII was 65% inhibited by iodo[14C]acetate and incorporated 0.6 mol of label into the α subunit, which eventually was allowed to form a precipitate. 2. All the label was recovered as S-carboxymethylcysteine in a tetra-peptide of sequence Gly-Gln-Cys-Trp.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chung S. I., Folk J. E. Kinetic studies with transglutaminases. The human blood enzymes (activated coagulation factor 13 and the guinea pig hair follicle enzyme. J Biol Chem. 1972 May 10;247(9):2798–2807. [PubMed] [Google Scholar]
  2. Crowshaw K., Jessup S. J., Ramwell P. W. Thin-layer chromatography of 1-dimethylaminonaphthalene-5-sulphonyl derivatives of amino acids present in superfusates of cat cerebral cortex. Biochem J. 1967 Apr;103(1):79–85. doi: 10.1042/bj1030079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Curtis C. G., Stenberg P., Chou C. H., Gray A., Brown K. L., Lorand L. Titration and subunit localization of active center cysteine in fibrinoligase (thrombin-activated fibrin stabilizing fector). Biochem Biophys Res Commun. 1973 May 1;52(1):51–56. doi: 10.1016/0006-291x(73)90952-2. [DOI] [PubMed] [Google Scholar]
  4. DALGLIESH C. E. The relation between pyridoxin and tryptophan metabolism, studied in the rat. Biochem J. 1952 Sep;52(1):3–14. doi: 10.1042/bj0520003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Folk J. E., Cole P. W. Identification of a functional cysteine essential for the activity of guinea pig liver transglutaminase. J Biol Chem. 1966 Jul 10;241(13):3238–3240. [PubMed] [Google Scholar]
  6. Folk J. E. Mechanism of action of guinea pig liver transglutaminase. VI. Order of substrate addition. J Biol Chem. 1969 Jul 10;244(13):3707–3713. [PubMed] [Google Scholar]
  7. LOEWY A. G., DUNATHAN K., GALLANT J. A., GARDNER B. Fibrinase III. Some enzymatic properties. J Biol Chem. 1961 Oct;236:2644–2647. [PubMed] [Google Scholar]
  8. LOEWY A. G., DUNATHAN K., KRIEL R., WOLFINGER H. L., Jr Fibrinase. I. Purification of substrate and enzyme. J Biol Chem. 1961 Oct;236:2625–2633. [PubMed] [Google Scholar]
  9. Lorand L., Downey J., Gotoh T., Jacobsen A., Tokura S. The transpeptidase system which crosslinks fibrin by gamma-glutamyle-episilon-lysine bonds. Biochem Biophys Res Commun. 1968 Apr 19;31(2):222–230. doi: 10.1016/0006-291x(68)90734-1. [DOI] [PubMed] [Google Scholar]
  10. Lorand L., Lockridge O. M., Campbell L. K., Myhrman R., Bruner-Lorand J. Transamidating enzymes. II. A continuous fluorescent method suited for automating measurements of factor XIII in plasma. Anal Biochem. 1971 Nov;44(1):221–231. doi: 10.1016/0003-2697(71)90363-0. [DOI] [PubMed] [Google Scholar]
  11. Matacić S., Loewy A. G. Transglutaminase activity of the fibrin crosslinking enzyme. Biochem Biophys Res Commun. 1966 Sep 22;24(6):858–866. doi: 10.1016/0006-291x(66)90327-5. [DOI] [PubMed] [Google Scholar]
  12. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  13. Pisano J. J., Finlayson J. S., Peyton M. P. [Cross-link in fibrin polymerized by factor 13: epsilon-(gamma-glutamyl)lysine]. Science. 1968 May 24;160(3830):892–893. doi: 10.1126/science.160.3830.892. [DOI] [PubMed] [Google Scholar]
  14. Schwartz M. L., Pizzo S. V., Hill R. L., McKee P. A. Human Factor XIII from plasma and platelets. Molecular weights, subunit structures, proteolytic activation, and cross-linking of fibrinogen and fibrin. J Biol Chem. 1973 Feb 25;248(4):1395–1407. [PubMed] [Google Scholar]
  15. Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]

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