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. 1973 Nov;136(3):697–703. doi: 10.1042/bj1360697

Quantitative studies on ferredoxin in greening bean leaves

B G Haslett 1,*, R Cammack 1, F R Whatley 2
PMCID: PMC1166005  PMID: 4360717

Abstract

Two methods of measuring small amounts of the iron–sulphur protein ferredoxin are described. One involves measurements of the signal at g=1.96 produced by reduced ferredoxin in an e.p.r. (electron-paramagnetic-resonance) spectrometer; the other depends on the rate of ferredoxin-dependent electron transport in a chloroplast bioassay measured in an O2 electrode. These methods of measurement were used to examine the development of ferredoxin during the greening of etiolated bean leaves. Ferredoxin is present in low concentrations in the leaves and cotyledons of 14-day-old etiolated beans (Phaseolus vulgaris L. var. Canadian Wonder), and develops in a linear manner with time when the leaves are illuminated. This synthesis appears to be independent of chlorophyll synthesis during the early stages of greening. However, the chlorophyll/ferredoxin ratio reaches a final value of approx. 360 irrespective of the light intensity, indicating the existence of a control mechanism operative in deciding the stoicheiometry of these components in the mature chloroplast. The ferredoxin synthesis appears to be light-dependent, and red light is the most effective in its promotion. The effect of red illumination is not reversed by far-red light, indicating the absence of a phytochrome control of ferredoxin synthesis. From experiments using specific inhibitors of chloroplast protein synthesis, it is concluded that ferredoxin is synthesized on cytoplasmic ribosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong J. J., Moll B., Surzycki S. J., Levine R. P. Genetic transcription and translation specifying chloroplast components in Chlamydomonas reinhardi. Biochemistry. 1971 Feb 16;10(4):692–701. doi: 10.1021/bi00780a022. [DOI] [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bottomley W., Spencer D., Wheeler A. M., Whitfeld P. R. The effect of a range of RNA polymerase inhibitors on RNA synthesis in higher plant chloroplasts and nuclei. Arch Biochem Biophys. 1971 Mar;143(1):269–275. doi: 10.1016/0003-9861(71)90209-8. [DOI] [PubMed] [Google Scholar]
  4. Ellis R. J., Hartley M. R. Sites of synthesis of chloroplast proteins. Nature. 1971 Oct 13;233(5320):193–196. [PubMed] [Google Scholar]
  5. Eytan G., Ohad I. Biogenesis of chloroplast membranes. 8. Modulation of chloroplast lamellae composition and function induced by discontinuous illumination and inhibition of ribonucleic acid and protein synthesis during greening of Chlamydomonas reinhardi y-1 mutant cells. J Biol Chem. 1972 Jan 10;247(1):122–129. [PubMed] [Google Scholar]
  6. Hartmann G., Honikel K. O., Knüsel F., Nüesch J. The specific inhibition of the DNA-directed RNA synthesis by rifamycin. Biochim Biophys Acta. 1967;145(3):843–844. doi: 10.1016/0005-2787(67)90147-5. [DOI] [PubMed] [Google Scholar]
  7. Hudock G. A., Levine R. P. Regulation of Photosynthesis in Chlamydomonas reinhardi. Plant Physiol. 1964 Nov;39(6):889–897. doi: 10.1104/pp.39.6.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Losada M., Paneque A. Light reduction of nitrate by chloroplasts depending on ferredoxin and NAD+. Biochim Biophys Acta. 1966 Nov 8;126(3):578–580. doi: 10.1016/0926-6585(66)90015-x. [DOI] [PubMed] [Google Scholar]
  9. Matsubara H. Puriication and some properties of Scenedesmus ferredoxin. J Biol Chem. 1968 Jan 25;243(2):370–375. [PubMed] [Google Scholar]
  10. TAGAWA K., ARNON D. I. Ferredoxins as electron carriers in photosynthesis and in the biological production and consumption of hydrogen gas. Nature. 1962 Aug 11;195:537–543. doi: 10.1038/195537a0. [DOI] [PubMed] [Google Scholar]
  11. Telfer Alison, Cammack R., Evans M. C.W. Hydrogen peroxide as the product of autoxidation of ferredoxin: Reduced either chemically or by illuminated chloroplasts. FEBS Lett. 1970 Sep 18;10(1):21–24. doi: 10.1016/0014-5793(70)80406-9. [DOI] [PubMed] [Google Scholar]
  12. WHATLEY F. R., TAGAWA K., ARNON D. I. Separation of the light and dark reactions in electron transfer during photosynthesis. Proc Natl Acad Sci U S A. 1963 Feb 15;49:266–270. doi: 10.1073/pnas.49.2.266. [DOI] [PMC free article] [PubMed] [Google Scholar]

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