Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1973 Nov;136(3):705–709. doi: 10.1042/bj1360705

Stimulation by vasopressin of glycogen breakdown and gluconeogenesis in the perfused rat liver

Douglas A Hems 1, Patricia D Whitton 1
PMCID: PMC1166006  PMID: 4780695

Abstract

1. Vasopressin (anti-diuretic hormone, [8-arginine]vasopressin) stimulated the breakdown of glycogen in perfused livers of fed rats, at concentrations (50–600μunits/ml) that have been reported in the blood of intact rats, especially during acute haemorrhagic shock. 2. In perfused livers from starved rats, vasopressin (30–150μunits/ml) stimulated gluconeogenesis from a mixture of lactate, pyruvate and glycerol. 3. Vasopressin prevented accumulation of liver glycogen in the perfused liver of starved rats, or in starved intact rats. 4. The action of vasopressin on hepatic carbohydrate metabolism thus resembles that of glucagon; the minimum effective circulating concentrations of these hormones are of the same order (100pg/ml). 5. The stimulation of hepatic glucose output by vasopressin is discussed in connexion with the release of glucose and water from the liver.

Full text

PDF
705

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERGEN S. S., Jr, SULLIVAN R., HILTON J. G., WILLIS S. W., Jr, VAN ITALLIE T. B. Glycogenolytic effect of vasopressin in the canine liver. Am J Physiol. 1960 Jul;199:136–138. doi: 10.1152/ajplegacy.1960.199.1.136. [DOI] [PubMed] [Google Scholar]
  2. Beck N. P., Kaneko T., Zor U., Field J. B., Davis B. B. Effects of vasopressin and prostaglandin E 1 on the adenyl cyclase-cyclic 3',5'-adenosine monophosphate system of the renal medulla of the rat. J Clin Invest. 1971 Dec;50(12):2461–2465. doi: 10.1172/JCI106746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burn J. H. The modification of the action of insulin by pituitary extract and other substances. J Physiol. 1923 Jun 8;57(5):318–329. doi: 10.1113/jphysiol.1923.sp002070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CASH W. D., KAPLAN M. H. A COMPARISON OF THE EFFECTS OF 8-LYSINE-VASOPRESSIN, 1-DEAMINO-8-LYSINE-VASOPRESSIN AND 1-ACETYL-8-LYSINE-VASOPRESSIN ON THE BLOOD GLUCOSE LEVEL IN RABBITS. Endocrinology. 1964 May;74:803–804. doi: 10.1210/endo-74-5-803. [DOI] [PubMed] [Google Scholar]
  5. Clark G. A. The origin of the glucose in the hyperglycaemia induced by pituitrin. J Physiol. 1928 Feb 10;64(4):324–330. doi: 10.1113/jphysiol.1928.sp002442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DEKANSKI J. The quantitative assay of vasopressin. Br J Pharmacol Chemother. 1952 Dec;7(4):567–572. doi: 10.1111/j.1476-5381.1952.tb00723.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Darnton S. J. Glycogen metabolism in rabbit kidney under differing physiological states. Q J Exp Physiol Cogn Med Sci. 1967 Oct;52(4):392–400. doi: 10.1113/expphysiol.1967.sp001933. [DOI] [PubMed] [Google Scholar]
  8. Exton J. H., Mallette L. E., Jefferson L. S., Wong E. H., Friedmann N., Miller T. B., Jr, Park C. R. The hormonal control of hepatic gluconeogenesis. Recent Prog Horm Res. 1970;26:411–461. doi: 10.1016/b978-0-12-571126-5.50014-5. [DOI] [PubMed] [Google Scholar]
  9. Forsling M. L., Jones J. J., Lee J. Factors influencing the sensitivity of the rat to vasopressin. J Physiol. 1968 May;196(2):495–505. doi: 10.1113/jphysiol.1968.sp008520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Forsling M. L., Martin M. J., Burton A. M. The effect of hydration on vasopressin and neurophysin release in the rat. J Endocrinol. 1971 Oct;51(2):413–414. doi: 10.1677/joe.0.0510413. [DOI] [PubMed] [Google Scholar]
  11. GINSBURG M., HELLER H. Antidiuretic activity in blood obtained from various parts of the cardiovascular system. J Endocrinol. 1953 Jul;9(3):274–282. doi: 10.1677/joe.0.0090274. [DOI] [PubMed] [Google Scholar]
  12. HEIDENREICH O., KOOK Y., BAUMEISTER L., KELLER P. [Studies on the mechanism of action of posterior pituitary hormones on carbohydrate and fat metabolism]. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1963;245:321–336. [PubMed] [Google Scholar]
  13. Hems D. A., Whitton P. D., Taylor E. A. Glycogen synthesis in the perfused liver of the starved rat. Biochem J. 1972 Sep;129(3):529–538. doi: 10.1042/bj1290529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Herrera E., Freinkel N. Interrelationships between liver composition, plasma glucose and ketones, and hepatic acetyl-CoA and citric acid during prolonged starvation in the male rat. Biochim Biophys Acta. 1968 Dec 23;170(2):244–253. doi: 10.1016/0304-4165(68)90004-4. [DOI] [PubMed] [Google Scholar]
  15. Imrie C. G. The action of extract of pituitary on the blood sugar after pancreatectomy. J Physiol. 1929 Jun 7;67(3):264–269. doi: 10.1113/jphysiol.1929.sp002567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnston I. D. The endocrine response to trauma. Adv Clin Chem. 1972;15:255–285. doi: 10.1016/s0065-2423(08)60161-4. [DOI] [PubMed] [Google Scholar]
  17. Krebs H. A., Dierks C., Gascoyne T. Carbohydrate synthesis from lactate in pigeon-liver homogenate. Biochem J. 1964 Oct;93(1):112–121. doi: 10.1042/bj0930112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lee E. Y., Whelan W. J. Enzymic methods for the microdetermination of glycogen and amylopectin, and their unit-chain lengths. Arch Biochem Biophys. 1966 Sep 26;116(1):162–167. doi: 10.1016/0003-9861(66)90024-5. [DOI] [PubMed] [Google Scholar]
  19. Little J. B., Klevay L. M., Radford E. P., Jr, McGandy R. B. Antidiuretic hormone inactivation by isolated perfused rat liver. Am J Physiol. 1966 Sep;211(3):786–792. doi: 10.1152/ajplegacy.1966.211.3.786. [DOI] [PubMed] [Google Scholar]
  20. MORTIMORE G. E. Effect of insulin on potassium transfer in isolated rat liver. Am J Physiol. 1961 Jun;200:1315–1319. doi: 10.1152/ajplegacy.1961.200.6.1315. [DOI] [PubMed] [Google Scholar]
  21. Pauk G. L., Reddy W. J. Evaluation of the liver adenosine 3',5'-monophosphate response to glucagon. Diabetes. 1971 Mar;20(3):129–133. doi: 10.2337/diab.20.3.129. [DOI] [PubMed] [Google Scholar]
  22. STEHLE R. L. The physiological actions of the hormones of the posterior lobe of the pituitary gland. Part 2. Actions other than those upon the circulation and the secretion of urine. Vitam Horm. 1950;8:215–254. [PubMed] [Google Scholar]
  23. Schillinger E., Loge O., Schröder E., Klieger E., Lübke K. Structure activity relationship of the insulin-like effects of the neurohypophysial peptide hormones. Eur J Biochem. 1972 Jun 9;27(3):473–481. doi: 10.1111/j.1432-1033.1972.tb01863.x. [DOI] [PubMed] [Google Scholar]
  24. Sokal J. E. Effect of glucagon on gluconeogenesis by the isolated perfused rat liver. Endocrinology. 1966 Mar;78(3):538–548. doi: 10.1210/endo-78-3-538. [DOI] [PubMed] [Google Scholar]
  25. Stumpf B., Boie A., Seubert W. In vitro stimulation of gluconeogenesis from pyruvate and of 14 CO 2 -fixation by dexamethasone phosphate and vasopressin in the kidney cortex of adrenalectomized rats. Biochem Biophys Res Commun. 1972 Oct 6;49(1):164–170. doi: 10.1016/0006-291x(72)90024-1. [DOI] [PubMed] [Google Scholar]
  26. Vaisler L., Costiner E., Gheorghiu C. Acţiunea hiperglicemiantă a oxitocinei în sistemul de ficat perfuzat in vitro. Stud Cercet Endocrinol. 1965;16(6):557–560. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES