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. 1973 Nov;136(3):731–740. doi: 10.1042/bj1360731

Rapid transport of phosphatidylcholine occurring simultaneously with protein transport in the frog sciatic nerve

Teruo Abe 1, Tatsuya Haga 1, Masanori Kurokawa 1
PMCID: PMC1166009  PMID: 4273556

Abstract

1. Either l-[4,5-3H]leucine or [Me-3H]choline, or both l-[U-14C]leucine and [Me-3H]-choline, were injected into the ninth dorsal root ganglion of the frog, and peripheral transport of labelled proteins and/or phospholipids, mostly phosphatidylcholine, was studied by analysis of consecutive segments of the sciatic nerve. 2. At 25°C, approx. 5% of the 3H-labelled protein was transported at the rate of 152mm/day. The rate was temperature-dependent with the Q10 value of 2.6. The flow was completely blocked by the local application of colchicine, but was unaffected by cytochalasin D. 3. [Me-3H]-Choline was incorporated into phosphatidylcholine at a comparatively slow rate, but was transported in the nerve at a rate equivalent to that for 3H-labelled proteins. 4. The simultaneous transport of phosphatidylcholine and the protein was further supported in the double-labelling experiments by an identical transport rate of 3H-labelled phosphatidylcholine and 14C-labelled proteins, by their identical temperature dependence, by simultaneous blockade with colchicine, and also by the parallel distribution of the two labels in subcellular fractions. Specific radioactivities on a protein basis of both 3H and 14C labels were highest in microsomal subfractions enriched with Na+-plus-K+-stimulated adenosine triphosphatase and acetylcholinesterase. It is suggested that 3H-labelled phosphatidylcholine and 14C-labelled proteins transported in the nerve reside in the same structural entity, most probably a membrane component.

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Selected References

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