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. 1973 Dec;136(4):829–836. doi: 10.1042/bj1360829a

Chloroplast ribosomal ribonucleic acid synthesis in cultured spinach leaf tissue

P Detchon 1,*, J V Possingham 1
PMCID: PMC1166029  PMID: 4786534

Abstract

Chloroplast rRNA synthesis was studied in spinach leaf tissue cultured under sterile conditions which eliminate bacterial rRNA synthesis. The synthesis was inhibited by darkness, but concomitant cytoplasmic rRNA synthesis was unaffected. A complex pattern of labelled rRNA precursors was found in extracts from cultured leaf tissue by using polyacrylamide-gel electrophoresis. However, differences between the precursor profiles of leaf tissue cultured in the light and in the dark could not be correlated with chloroplast rRNA synthesis since large amounts of high-molecular-weight precursors of cytoplasmic rRNA dominated the pattern in both cases. A double-isotope-labelling technique was used, which enabled light-stimulated rRNA synthesis to be studied in whole leaf tissue. Two rapidly labelled RNA species of molecular weights 1.15×106 and 0.65×106 were detected, which were thought to have possible precursor significance in the synthesis of mature chloroplast rRNA of molecular weights 1.04×106 and 0.56×106 respectively. Cycloheximide treatment resulted in the accumulation of RNA of molecular weight 1.8×106, whose function is unknown.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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