Abstract
A new method is described for plotting kinetic results for inhibited enzyme-catalysed reactions. It provides a simple way of determining the inhibition constant, K′i, of an uncompetitive, mixed or non-competitive inhibitor.
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Selected References
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- DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOWD J. E., RIGGS D. S. A COMPARISON OF ESTIMATES OF MICHAELIS-MENTEN KINETIC CONSTANTS FROM VARIOUS LINEAR TRANSFORMATIONS. J Biol Chem. 1965 Feb;240:863–869. [PubMed] [Google Scholar]
- JOHANSEN G., LUMRY R. Statistical analysis of enzymic steady-state rate data. C R Trav Lab Carlsberg. 1961;32:185–214. [PubMed] [Google Scholar]
- Schlamowitz M., Shaw A., Jackson W. T. Limitations of he Dixon plot for ascertaining naure of enzyme inhibition. Tex Rep Biol Med. 1969 Summer;27(2):483–488. [PubMed] [Google Scholar]
- WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]