Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1974 Feb;137(2):413–415. doi: 10.1042/bj1370413

Fluorescence studies of protein–sterol relationships in human plasma lipoproteins (Short Communication)

Rory J M Smith 1, Colin Green 1
PMCID: PMC1166131  PMID: 4363114

Abstract

Cholesta-5,7,9(11)-trien-3β-ol and its oleate ester were incorporated into human low-density lipoprotein and reconstituted high-density lipoprotein. The unesterified sterol was more efficient than its ester in quenching tryptophan fluorescence, especially in low-density lipoprotein. The results, which indicate that in such lipoproteins unesterified sterols are more closely associated with peptide than are esterified sterols, are used to assess possible structures for the lipoproteins.

Full text

PDF
413

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHWORTH L. A., GREEN C. The uptake of lipids by human alpha-lipoprotein. Biochim Biophys Acta. 1963 Feb 19;70:68–74. doi: 10.1016/0006-3002(63)90719-4. [DOI] [PubMed] [Google Scholar]
  2. Brocklehurst J. R., Freedman R. B., Hancock D. J., Radda G. K. Membrane studies with polarity-dependent and excimer-forming fluorescent probes. Biochem J. 1970 Feb;116(4):721–731. doi: 10.1042/bj1160721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Day C. E., Levy R. S. Molecular structure of serum lipoproteins. J Theor Biol. 1969 Jun;23(3):387–399. doi: 10.1016/0022-5193(69)90027-7. [DOI] [PubMed] [Google Scholar]
  4. Forte T. M., Nichols A. V., Gong E. L., Levy R. I., Lux S. Electron microscopic study on reassembly of plasma high density apoprotein with various lipids. Biochim Biophys Acta. 1971 Nov 5;248(2):381–386. doi: 10.1016/0005-2760(71)90026-9. [DOI] [PubMed] [Google Scholar]
  5. Forte T., Norum K. R., Glomset J. A., Nichols A. V. Plasma lipoproteins in familial lecithin: cholesterol acyltransferase deficiency: structure of low and high density lipoproteins as revealed by elctron microscopy. J Clin Invest. 1971 May;50(5):1141–1148. doi: 10.1172/JCI106586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Laggner P., Müller K., Kratky O., Kostner G., Holasek A. Studies on the structure of lipoprotein A of human high density lipoprotein HDL3: the spherically averaged electron density distribution. FEBS Lett. 1973 Jun 15;33(1):77–80. doi: 10.1016/0014-5793(73)80163-2. [DOI] [PubMed] [Google Scholar]
  8. Margolis S., Langdon R. G. Studies on human serum beta-1-lipoprotein. 3. Enzymatic modifications. J Biol Chem. 1966 Jan 25;241(2):485–493. [PubMed] [Google Scholar]
  9. Mateu L., Tardieu A., Luzzati V., Aggerbeck L., Scanu A. M. On the structure of human serum low density lipoprotein. J Mol Biol. 1972 Sep 14;70(1):105–116. doi: 10.1016/0022-2836(72)90166-0. [DOI] [PubMed] [Google Scholar]
  10. Pollard H. B., Chen R. F. Fluorescence and circular dichroism studies on human serum low density lipoprotein particles and lipid-depleted derivatives. J Supramol Struct. 1973;1(3):177–184. doi: 10.1002/jss.400010303. [DOI] [PubMed] [Google Scholar]
  11. Pollard H., Scanu A. M., Taylor E. W. On the geometrical arrangement of the protein subunits of human serum low-density lipoprotein: evidence for a dodecahedral model. Proc Natl Acad Sci U S A. 1969 Sep;64(1):304–310. doi: 10.1073/pnas.64.1.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scanu A. M., Edelstein C. Solubility in aqueous solutions of ethanol of the small molecular weight peptides of the serum very low density and high density lipoproteins: relevance to the recovery problem during delipidation of serum lipoproteins. Anal Biochem. 1971 Dec;44(2):576–588. doi: 10.1016/0003-2697(71)90247-8. [DOI] [PubMed] [Google Scholar]
  13. Scanu A. Forms of human serum high density lipoprotein protein. J Lipid Res. 1966 Mar;7(2):295–306. [PubMed] [Google Scholar]
  14. Schneider H., Morrod R. S., Colvin J. R., Tattrie N. H. The lipid core model of lipoproteins. Chem Phys Lipids. 1973 May;10(4):328–353. doi: 10.1016/0009-3084(73)90058-3. [DOI] [PubMed] [Google Scholar]
  15. Shipley G. G., Atkinson D., Scanu A. M. Small-angle x-ray scattering of human serum high-density lipoproteins. J Supramol Struct. 1972;1(2):98–104. doi: 10.1002/jss.400010203. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES