Figure 5. Serum ADA2 enzymatic activity of suspected DADA2 patients.

A. ADA2 enzyme activity (U/L) measured in serum samples of suspected DADA2 patients (n=10), adult healthy controls (n=35), pediatric healthy controls (n=17), healthy DADA2 carriers (n=19) and DADA2 patients (n=18). Each data point is plotted with mean ± SD. Statistical significance was assessed using the Mann-Whitney U test,*** p<0.0001. B. ADA2 protein secretion in Serum samples of pediatric controls, adult controls, DADA2 patients and cohort patients by Western blot. C-D. Quantification of ADA2 secretion in Serum samples of pediatric controls, adult controls, DADA2 patients and cohort patients. Bar graphs represent percentage of ADA2 protein secretion relative to Pediatric/adult controls. Each bar represents mean ± SD from 3 independent experiments.