Figure 4. Using traditional serial section for imaging of mandible of Pdgfra^CreER×Nfatc1^DreER× LGRT mice (pulse).

(A) Schematic illustration of lineaging tracing in Pdgfra^CreER×Nfatc1^DreER× LGRT mice. (B) Operation process of frozen section. Using this procedure, a total of 88 slices were collected in this sample. (C) Mandible M1 after 3D reconstruction by Imaris, including pulp and PDL with virtual dentin shell (white) in buccal view, coronal view and radicular view (scale bar = 200 μm). (D) 3D reconstruction of mandible M1 by DICOM-3D; in PDL, ZsGreen⁺ cells in green, tdTomato⁺ cells in rose red; in pulp, ZsGreen⁺ cells in purple, tdTomato⁺ cells in blue. The image stack was also displayed in buccal view, coronal view, and radicular view of pulp and PDL, respectively (D-b), scale bar = 200 μm. (D-a): The legend of (D-b).

Figure 4—figure supplement 1. The total 88 slices of mandible M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice sample (pulse).

The images were acquired by confocal microscope, ZsGreen⁺ cells in green, tdTomato⁺ cells in red, DAPI in blue.

Figure 4—figure supplement 2. Representative images of coronal pulp (A) and PDL (B) acquired by confocal microscope of mandible M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice sample (pulse).

The images were acquired by confocal microscope (Scale bar = 100 μm).

Figure 4—video 1. Panoptic multicolor imaging of PDGFR-α⁺ cells & NFATc1⁺ cells in the pulp and PDL area of mandible M1 of Pdgfra^CreER×Nfatc1^DreER× LGRT mice (pulse), the whole-tissue imaging was reconstructed from serial sections, related to Figure 4C.

Download video file ^{(4.9MB, mp4)}

PDGFR-α⁺ cells in green, NFATc1⁺ cells in red and DAPI in blue.