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. 1974 Feb;138(2):239–252. doi: 10.1042/bj1380239

Accumulation of lanthanum by rat liver mitochondria

Ken C Reed 1, Fyfe L Bygrave 1
PMCID: PMC1166201  PMID: 4362742

Abstract

The interaction of La3+ with rat liver mitochondria was examined with a wide variety of techniques permitting measurement of respiratory and structural responses. It is concluded that La3+ is accumulated by mitochondria in a process that is at least partially energy-dependent, and is bound with quite high affinity to membrane-associated sites both external and internal to the inner membrane. The relative insensitivity of the accumulation process to respiratory inhibitors and to the permeant anion acetate has interesting implications for the mechanism of active cation transport.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAGINSKI E., ZAK B. Micro-determination of serum phosphate and phospholipids. Clin Chim Acta. 1960 Nov;5:834–838. doi: 10.1016/0009-8981(60)90117-0. [DOI] [PubMed] [Google Scholar]
  2. BRADLEY L. B., JACOB M., JACOBS E. E., SANADI D. R. Uncoupling of oxidative phosphorylation by cadmium ion. J Biol Chem. 1956 Nov;223(1):147–156. [PubMed] [Google Scholar]
  3. Bielawski J., Lehninger A. L. Stoichiometric relationships in mitochondrial accumulation of calcium and phosphate supported by hydrolysis of adenosine triphosphate. J Biol Chem. 1966 Oct 10;241(19):4316–4322. [PubMed] [Google Scholar]
  4. CHANCE B. THE ENERGY-LINKED REACTION OF CALCIUM WITH MITOCHONDRIA. J Biol Chem. 1965 Jun;240:2729–2748. [PubMed] [Google Scholar]
  5. CHANCE B., WILLIAMS G. R. The respiratory chain and oxidative phosphorylation. Adv Enzymol Relat Subj Biochem. 1956;17:65–134. doi: 10.1002/9780470122624.ch2. [DOI] [PubMed] [Google Scholar]
  6. CHAPPELL J. B., CROFTS A. R. CALCIUM ION ACCUMULATION AND VOLUME CHANGES OF ISOLATED LIVER MITOCHONDRIA. CALCIUM ION-INDUCED SWELLING. Biochem J. 1965 May;95:378–386. doi: 10.1042/bj0950378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chance B., Azzi A. The response of reduced pyridine nucleotide to calcium-induced alkalinity. Ann N Y Acad Sci. 1969 Oct 31;147(19):805–811. doi: 10.1111/j.1749-6632.1969.tb41287.x. [DOI] [PubMed] [Google Scholar]
  8. Chance B., Ernster L., Garland P. B., Lee C. P., Light P. A., Ohnishi T., Ragan C. I., Wong D. Flavoproteins of the mitochondrial respiratory chain. Proc Natl Acad Sci U S A. 1967 May;57(5):1498–1505. doi: 10.1073/pnas.57.5.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chance B., Schoener B. High and low energy states of cytochromes. 3. In reactions with cations. J Biol Chem. 1966 Oct 25;241(20):4577–4587. [PubMed] [Google Scholar]
  10. GREENAWALT J. W., ROSSI C. S., LEHNINGER A. L. EFFECT OF ACTIVE ACCUMULATION OF CALCIUM AND PHOSPHATE IONS ON THE STRUCTURE OF RAT LIVER MITOCHONDRIA. J Cell Biol. 1964 Oct;23:21–38. doi: 10.1083/jcb.23.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Greenawalt J. W., Carafoli E. Electron microscope studies on the active accumulation of Sr++ by rat-liver mitochondria. J Cell Biol. 1966 Apr;29(1):37–61. doi: 10.1083/jcb.29.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hatefi Y. Flavoproteins of the electron transport system and the site of action of amytal, rotenone, and piericidin A. Proc Natl Acad Sci U S A. 1968 Jun;60(2):733–740. doi: 10.1073/pnas.60.2.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LEHNINGER A. L. Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation. Physiol Rev. 1962 Jul;42:467–517. doi: 10.1152/physrev.1962.42.3.467. [DOI] [PubMed] [Google Scholar]
  14. Lehninger A. L., Carafoli E. The interaction of La 3+ with mitochondria in relation to respiration-coupled Ca 2+ transport. Arch Biochem Biophys. 1971 Apr;143(2):506–515. doi: 10.1016/0003-9861(71)90235-9. [DOI] [PubMed] [Google Scholar]
  15. Lemberg M. R. Cytochrome oxidase. Physiol Rev. 1969 Jan;49(1):48–121. doi: 10.1152/physrev.1969.49.1.48. [DOI] [PubMed] [Google Scholar]
  16. Mela L., Chance B. Calcium carrier and the "high affinity calcium binding site" in mitochondria. Biochem Biophys Res Commun. 1969 May 22;35(4):556–559. doi: 10.1016/0006-291x(69)90383-0. [DOI] [PubMed] [Google Scholar]
  17. Mela L., Chance B. Spectrophotometric measurements of the kinetics of Ca2+ and Mn2+ accumulation in mitochondria. Biochemistry. 1968 Nov;7(11):4059–4063. doi: 10.1021/bi00851a038. [DOI] [PubMed] [Google Scholar]
  18. Mela L. Inhibition and activation of calcium transport in mitochondria. Effect of lanthanides and local anesthetic drugs. Biochemistry. 1969 Jun;8(6):2481–2486. doi: 10.1021/bi00834a034. [DOI] [PubMed] [Google Scholar]
  19. Mela L. Interactions of La3+ and local anesthetic drugs with mitochondrial Ca++ and Mn++ uptake. Arch Biochem Biophys. 1968 Feb;123(2):286–293. doi: 10.1016/0003-9861(68)90136-7. [DOI] [PubMed] [Google Scholar]
  20. Mela L. Reaction of lanthanides with mitochondrial membranes. Ann N Y Acad Sci. 1969 Oct 31;147(19):824–828. doi: 10.1111/j.1749-6632.1969.tb41290.x. [DOI] [PubMed] [Google Scholar]
  21. Myron D. R., Connelly J. L. The morphology of the swelling process in rat liver mitochondria. J Cell Biol. 1971 Feb;48(2):291–302. doi: 10.1083/jcb.48.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ROSSI C. S., LEHNINGER A. L. STOICHIOMETRY OF RESPIRATORY STIMULATION, ACCUMULATION OF CA++ AND PHOSPHATE, AND OXIDATIVE PHOSPHORYLATION IN RAT LIVER MITOCHONDRIA. J Biol Chem. 1964 Nov;239:3971–3980. [PubMed] [Google Scholar]
  23. Reed K. C. An oxygen polarograph designed for undergraduate use. Anal Biochem. 1972 Nov;50(1):206–212. doi: 10.1016/0003-2697(72)90500-3. [DOI] [PubMed] [Google Scholar]
  24. Reynafarje B., Lehninger A. L. High affinity and low affinity binding of Ca++ by rat liver mitochondria. J Biol Chem. 1969 Feb 25;244(4):584–593. [PubMed] [Google Scholar]
  25. SZARKOWSKA L., KLINGENBERG M. ON THE ROLE OF UBIQUINONE IN MITOCHONDRIA. SPECTROPHOTOMETRIC AND CHEMICAL MEASUREMENTS OF ITS REDOX REACTIONS. Biochem Z. 1963;338:674–697. [PubMed] [Google Scholar]
  26. Sato T. A modified method for lead staining of thin sections. J Electron Microsc (Tokyo) 1968;17(2):158–159. [PubMed] [Google Scholar]
  27. Scarpa A., Azzone G. F. The mechanism of ion translocation in mitochondria. 4. Coupling of K+ efflux with Ca2+ uptake. Eur J Biochem. 1970 Feb;12(2):328–335. doi: 10.1111/j.1432-1033.1970.tb00854.x. [DOI] [PubMed] [Google Scholar]
  28. Selwyn M. J., Dawson A. P., Dunnett S. J. Calcium transport in mitochondria. FEBS Lett. 1970 Sep 18;10(1):1–5. doi: 10.1016/0014-5793(70)80402-1. [DOI] [PubMed] [Google Scholar]
  29. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  30. Stoner C. D., Sirak H. D. Osmotically-induced alterations in volume and ultrastructure of mitochondria isolated from rat liver and bovine heart. J Cell Biol. 1969 Dec;43(3):521–538. doi: 10.1083/jcb.43.3.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Vainio H., Mela L., Chance B. Energy dependent bivalent cation translocation in rat liver mitochondria. Eur J Biochem. 1970 Feb;12(2):387–391. doi: 10.1111/j.1432-1033.1970.tb00863.x. [DOI] [PubMed] [Google Scholar]
  32. Vinogradov A., Scarpa A., Chance B. Calcium and pyridine nucleotide interaction in mitochondrial membranes. Arch Biochem Biophys. 1972 Oct;152(2):646–654. doi: 10.1016/0003-9861(72)90261-5. [DOI] [PubMed] [Google Scholar]

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