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. 1974 Apr;139(1):157–162. doi: 10.1042/bj1390157

Fractionation of nucleic acids from Penicillium chrysogenum and associated ribonucleic acid viruses by selective exclusion and retention in agarose gels

S L Petrović 1, B D Šumonja 1, R B Vasiljević 1
PMCID: PMC1166262  PMID: 4219136

Abstract

Double-stranded nucleic acids from a strain of Penicillium chrysogenum containing RNA viruses were isolated by agarose-gel filtration, and separated into DNA and double-stranded RNA fractions by agarose-gel chromatography in 2.5m-NaCl. The DNA fraction contained less than 1% alkali-labile polynucleotides, and sedimented homogeneously at 8–10S in alkaline sucrose gradients. In CsCl gradients it tended to band in the density region of 1.66–1.72g/ml. It had a `melting' temperature (Tm) of 75°C in 0.015m-NaCl–0.0015m-trisodium citrate, corresponding to 51.5mol% of G+C. The double-stranded RNA fraction did not contain detectable DNA. It could not band in CsCl up to a density of 1.78g/ml, and mainly consisted of a 14–15S RNA species with a Tm of 88.5°C in the above solvent, and a G+C content of 49.3 mol%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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