Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1974 Jun;139(3):509–513. doi: 10.1042/bj1390509

Structural membrane proteins and loosely associated proteins of the sarcoplasmic reticulum

A Margreth 1, U Carraro 1, G Salviati 1
PMCID: PMC1166315  PMID: 4369219

Abstract

The protein composition of sarcoplasmic-reticulum vesicles, either unpurified or after fractionation on sucrose gradients, and with or without previous osmotic shock and sonication, was investigated by electrophoresis in acid polyacrylamide gels. The pattern of release of loosely bound proteins is discussed with respect to their localization in the interior of the vesicles.

Full text

PDF
509

Images in this article

510-2PLATE 2
on p.510-2
510-1PLATE 1
on p.510-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Darnall D. W., Klotz I. M. Protein subunits: a table (revised edition). Arch Biochem Biophys. 1972 Mar;149(1):1–14. doi: 10.1016/0003-9861(72)90293-7. [DOI] [PubMed] [Google Scholar]
  2. Duggan P. F., Martonosi A. Sarcoplasmic reticulum. IX. The permeability of sarcoplasmic reticulum membranes. J Gen Physiol. 1970 Aug;56(2):147–167. doi: 10.1085/jgp.56.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ikemoto N., Bhatnagar G. M., Nagy B., Gergely J. Interaction of divalent cations with the 55,000-dalton protein component of the sarcoplasmic reticulum. Studies of fluorescence and circular dichroism. J Biol Chem. 1972 Dec 10;247(23):7835–7837. [PubMed] [Google Scholar]
  4. Inesi G., Maring E., Murphy A. J., McFarland B. H. A study of the phosphorylated intermediate of sarcoplasmic reticulum ATPase. Arch Biochem Biophys. 1970 May;138(1):285–294. doi: 10.1016/0003-9861(70)90309-7. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Leonard K. R., Walker I. O. The self-association of rabbit-muscle phosphofructokinase. Eur J Biochem. 1972 Apr 11;26(3):442–448. doi: 10.1111/j.1432-1033.1972.tb01785.x. [DOI] [PubMed] [Google Scholar]
  7. MacLennan D. H. Purification and properties of an adenosine triphosphatase from sarcoplasmic reticulum. J Biol Chem. 1970 Sep 10;245(17):4508–4518. [PubMed] [Google Scholar]
  8. MacLennan D. H., Seeman P., Iles G. H., Yip C. C. Membrane formation by the adenosine triphosphatase of sarcoplasmic reticulum. J Biol Chem. 1971 Apr 25;246(8):2702–2710. [PubMed] [Google Scholar]
  9. MacLennan D. H., Wong P. T. Isolation of a calcium-sequestering protein from sarcoplasmic reticulum. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1231–1235. doi: 10.1073/pnas.68.6.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Margreth A., Salviati G., Catani C. Electron transport in sarcoplasmic reticulum of fast and slow muscles. Arch Biochem Biophys. 1971 Jun;144(2):768–772. doi: 10.1016/0003-9861(71)90385-7. [DOI] [PubMed] [Google Scholar]
  11. Margreth A., Salviati G., Di Mauro S., Turati G. Early biochemical consequences of denervation in fast and slow skeletal muscles and their relationship to neural control over muscle differentiation. Biochem J. 1972 Mar;126(5):1099–1110. doi: 10.1042/bj1261099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Martonosi A., Halpin R. A. Sarcoplasmic reticulum. X. The protein composition of sarcoplasmic reticulum membranes. Arch Biochem Biophys. 1971 May;144(1):66–77. doi: 10.1016/0003-9861(71)90455-3. [DOI] [PubMed] [Google Scholar]
  13. Martonosi A. Sarcoplasmic reticulum. IV. Solubilization of microsomal adenosine triphosphatase. J Biol Chem. 1968 Jan 10;243(1):71–81. [PubMed] [Google Scholar]
  14. Meissner G., Conner G. E., Fleischer S. Isolation of sarcoplasmic reticulum by zonal centrifugation and purification of Ca 2+ -pump and Ca 2+ -binding proteins. Biochim Biophys Acta. 1973 Mar 16;298(2):246–269. doi: 10.1016/0005-2736(73)90355-6. [DOI] [PubMed] [Google Scholar]
  15. Panet R., Selinger Z. Specific alkylation of the sarcoplasmic reticulum ATPase by N-ethyl-[1-14C]maleimide and identification of the labeled protein in acrylamide gel-electrophoresis. Eur J Biochem. 1970 Jul;14(3):440–444. doi: 10.1111/j.1432-1033.1970.tb00308.x. [DOI] [PubMed] [Google Scholar]
  16. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES