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. 1974 Jun;139(3):645–652. doi: 10.1042/bj1390645

A kinetic evaluation of monoamine oxidase activity in rat liver mitochondrial outer membranes

Miles D Houslay 1, Keith F Tipton 1
PMCID: PMC1166328  PMID: 4850215

Abstract

1. A preparation of mitochondrial outer membranes from rat liver can be shown to contain two kinetically distinct monoamine oxidase activities. These activities are distinguishable by their different sensitivities to the irreversible inhibitor clorgyline, and by the effect of the reversible inhibitors benzyl cyanide and 4-cyanophenol. 2. The substrate specificities of the preparation and the two enzyme species have been elucidated.

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Selected References

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  1. Blaschko H., Richter D., Schlossmann H. The oxidation of adrenaline and other amines. Biochem J. 1937 Dec;31(12):2187–2196. doi: 10.1042/bj0312187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Collins G. G., Sandler M., Williams E. D., Youdim M. B. Multiple forms of human brain mitochondrial monoamine oxidase. Nature. 1970 Feb 28;225(5235):817–820. doi: 10.1038/225817a0. [DOI] [PubMed] [Google Scholar]
  3. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GORKIN V. Z. PARTIAL SEPARATION OF RAT LIVER MITOCHONDRIAL AMINE OXIDASES. Nature. 1963 Oct 5;200:77–77. doi: 10.1038/200077a0. [DOI] [PubMed] [Google Scholar]
  5. Gross M., Folk J. E. Mapping of the active sites of transglutaminases. II. Activity of the guinea pig liver enzyme toward methylglutamine peptide derivatives. J Biol Chem. 1973 Sep 25;248(18):6534–6542. [PubMed] [Google Scholar]
  6. Hall D. W., Logan B. W., Parsons G. H. Further studies on the inhibition of monoamine oxidase by M and B 9302 (clorgyline). I. Substrate specificity in various mammalian species. Biochem Pharmacol. 1969 Jun;18(6):1447–1454. doi: 10.1016/0006-2952(69)90258-5. [DOI] [PubMed] [Google Scholar]
  7. Houslay M. D., Tipton K. F. The nature of the electrophoretically separable multiple forms of rat liver monoamine oxidase. Biochem J. 1973 Sep;135(1):173–186. doi: 10.1042/bj1350173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Houslay M. D., Tipton K. F. The reaction pathway of membrane-bound rat liver mitochondrial monoamine oxidase. Biochem J. 1973 Dec;135(4):735–750. doi: 10.1042/bj1350735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jarrott B. Occurrence and properties of monoamine oxidase in adrenergic neurons. J Neurochem. 1971 Jan;18(1):7–16. doi: 10.1111/j.1471-4159.1971.tb00162.x. [DOI] [PubMed] [Google Scholar]
  10. Johnston J. P. Some observations upon a new inhibitor of monoamine oxidase in brain tissue. Biochem Pharmacol. 1968 Jul;17(7):1285–1297. doi: 10.1016/0006-2952(68)90066-x. [DOI] [PubMed] [Google Scholar]
  11. KOBAYASHI Y., SCHAYER R. W. Action of rat liver monoamine oxidase on multiple substrates. Arch Biochem Biophys. 1955 Sep;58(1):181–188. doi: 10.1016/0003-9861(55)90104-1. [DOI] [PubMed] [Google Scholar]
  12. Kohn H. I. Tyramine oxidase. Biochem J. 1937 Oct;31(10):1693–1704. doi: 10.1042/bj0311693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kroon M. C., Veldstra H. Multiple forms of rat brain mitochondrial monoamine oxidase. Subcellular localization. FEBS Lett. 1972 Aug 1;24(2):173–176. doi: 10.1016/0014-5793(72)80760-9. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. McEwen C. M., Jr, Sasaki G., Jones D. C. Human liver mitochondrial monoamine oxidase. 3. Kinetic studies concerning time-dependent inhibitions. Biochemistry. 1969 Oct;8(10):3963–3972. doi: 10.1021/bi00838a012. [DOI] [PubMed] [Google Scholar]
  16. McEwen C. M., Jr, Sasaki G., Lenz W. R., Jr Human liver mitochondrial monoamine oxidase. I. Kinetic studies of model interactions. J Biol Chem. 1968 Oct 25;243(20):5217–5225. [PubMed] [Google Scholar]
  17. Neff N. H., Goridis C. Neuronal monoamine oxidase: specific enzyme types and their rates of formation. Adv Biochem Psychopharmacol. 1972;5:307–323. [PubMed] [Google Scholar]
  18. OSWALD E. O., STRITTMATTER C. F. COMPARATIVE STUDIES IN THE CHARACTERIZATION OF MONOAMINE OXIDASES. Proc Soc Exp Biol Med. 1963 Dec;114:668–673. doi: 10.3181/00379727-114-28765. [DOI] [PubMed] [Google Scholar]
  19. Pugh C. E., Quastel J. H. Oxidation of amines by animal tissues. Biochem J. 1937 Dec;31(12):2306–2321. doi: 10.1042/bj0312306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sharman D. F. The catabolism of catecholamines. Recent studies. Br Med Bull. 1973 May;29(2):110–115. doi: 10.1093/oxfordjournals.bmb.a070978. [DOI] [PubMed] [Google Scholar]
  21. Sierens L., D'Iorio A. Multiple monoamine oxidases in rat liver mitochondria. Can J Biochem. 1970 Jun;48(6):659–663. doi: 10.1139/o70-105. [DOI] [PubMed] [Google Scholar]
  22. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Squires R. F. Multiple forms of monoamine oxidase in intact mitochondria as characterized by selective inhibitors and thermal stability: a comparison of eight mammalian species. Adv Biochem Psychopharmacol. 1972;5:355–370. [PubMed] [Google Scholar]
  24. TABOR C. W., TABOR H., ROSENTHAL S. M. Purification of amine oxidase from beef plasma. J Biol Chem. 1954 Jun;208(2):645–661. [PubMed] [Google Scholar]
  25. Tipton K. F., Houslay M. D., Garrett N. J. Allotopic properties of human brain monoamine oxidase. Nat New Biol. 1973 Dec 19;246(155):213–214. doi: 10.1038/newbio246213a0. [DOI] [PubMed] [Google Scholar]
  26. Tipton K. F. Some properties of monoamine oxidase. Adv Biochem Psychopharmacol. 1972;5:11–24. [PubMed] [Google Scholar]
  27. Tipton K. F., Spires I. P. The kinetics of phenethylhydrazine oxidation by monoamine oxidase. Biochem J. 1971 Nov;125(2):521–524. doi: 10.1042/bj1250521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Youdim M. B. Multiple forms of mitochondrial monoamine oxidase. Br Med Bull. 1973 May;29(2):120–122. doi: 10.1093/oxfordjournals.bmb.a070980. [DOI] [PubMed] [Google Scholar]
  29. Youdim M. B., Sourkes T. L. The flavin prosthetic group of purified rat liver mitochondrial monoamine oxidase. Adv Biochem Psychopharmacol. 1972;5:45–53. [PubMed] [Google Scholar]

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