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. 1974 Jun;139(3):771–777. doi: 10.1042/bj1390771

Purification and subunit analysis of wheat-germ ribonucleic acid polymerase II

Jerome J Jendrisak 1, Wayne M Becker 1
PMCID: PMC1166341  PMID: 4853970

Abstract

A procedure is described for the purification of the α-amanitin-sensitive DNA-dependent RNA polymerase [EC 2.7.7.6] from wheat germ. Solubilization of the enzyme activity was achieved by sonication of a crude extract in a high-salt buffer. Purification involved precipitation with protamine sulphate and (NH4)2SO4, chromatography on DEAE-cellulose and phosphocellulose, and sucrose gradient centrifugation. Under denaturing conditions the enzyme dissociated into five polypeptides with molecular weights and molar ratios of 220000 (0.9), 170000 (0.1), 140000 (1.0), 45000 (0.2), and 40000 (0.4). Approx. 1mg of purified RNA polymerase was obtained as a routine from 100g of starting material.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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