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. 1986 Jun;146:131–139.

Ultrastructural visualisation of carbohydrate groups in the surface coating of hamster alveolar macrophages and pneumonocytes.

C Meban 1
PMCID: PMC1166529  PMID: 2447050

Abstract

The surface coating of the alveolar macrophages and pneumonocytes of hamster lung was studied using an electron microscopy technique. Slices of lung tissue were fixed in aldehyde, labelled with a battery of lectin-horseradish peroxidase conjugates, incubated in a diaminobenzidine-hydrogen peroxide medium and then postfixed in an osmium tetroxide solution. The results of the study suggest that the surface coating of the pneumonocytes and macrophages contains the following carbohydrate groups: N-acetylgalactosamine, N-acetylglucosamine, D-mannose, L-fucose, D-galactose and sialic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamson I. Y., Bowden D. H. The surface complexes of the lung. A cytochemical partition of phospholipid surfactant and mucopolysaccharide. Am J Pathol. 1970 Dec;61(3):359–376. [PMC free article] [PubMed] [Google Scholar]
  2. Bignon J., Jaubert F., Jaurand M. C. Plasma protein immunocytochemistry and polysaccharide cytochemistry at the surface of alveolar and endothelial cells in the rat lung. J Histochem Cytochem. 1976 Oct;24(10):1076–1084. doi: 10.1177/24.10.789758. [DOI] [PubMed] [Google Scholar]
  3. GRONIOWSKI J., BICZYSKOWA W. STRUCTURE OF THE ALVEOLAR LINING FILM OF THE LUNGS. Nature. 1964 Nov 21;204:745–747. doi: 10.1038/204745a0. [DOI] [PubMed] [Google Scholar]
  4. Goldstein I. J., Hayes C. E. The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem. 1978;35:127–340. doi: 10.1016/s0065-2318(08)60220-6. [DOI] [PubMed] [Google Scholar]
  5. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  6. Gros D., Obrénovitch A., Challice C. E., Monsigny M., Schrével J. Ultrastructural visualization of cellular carbohydrate components by means of lectins on ultrathin glycol methacrylate sections. J Histochem Cytochem. 1977 Feb;25(2):104–114. doi: 10.1177/25.2.65417. [DOI] [PubMed] [Google Scholar]
  7. Guillouzo A., Feldmann G. Surface and intracellular localization of concanavalin A binding sites in rat liver cells. J Histochem Cytochem. 1977 Dec;25(12):1303–1310. doi: 10.1177/25.12.336784. [DOI] [PubMed] [Google Scholar]
  8. Hardham A. R. Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding. J Histochem Cytochem. 1985 Feb;33(2):110–118. doi: 10.1177/33.2.3918095. [DOI] [PubMed] [Google Scholar]
  9. Katsuyama T., Spicer S. S. A cation-retaining layer in the alveolar-capillary membrane. Lab Invest. 1977 Apr;36(4):428–435. [PubMed] [Google Scholar]
  10. Kikkawa Y., Hahn H. S., Yang S. S., Bernstein J. Mucopolysaccharide in the pulmonary alveolus. II. Electron microscopic observations. Lab Invest. 1970 Mar;22(3):272–280. [PubMed] [Google Scholar]
  11. Meban C. An electron microscopic study of the acid mucosubstance lining the alveoli of hamster lung. Histochem J. 1972 Jan;4(1):1–8. doi: 10.1007/BF01005264. [DOI] [PubMed] [Google Scholar]
  12. Meban C. The carbohydrates associated with the surfaces of hamster alveolar macrophages: a study using fluorescein-conjugated lectins. J Anat. 1985 Mar;140(Pt 2):221–228. [PMC free article] [PubMed] [Google Scholar]
  13. Meban C. The surface coating of the pneumonocytes in human neonatal lung. J Anat. 1984 Sep;139(Pt 2):371–385. [PMC free article] [PubMed] [Google Scholar]
  14. Miller R. L., Cannon J. D., Jr Limax flavus agglutinin: properties of a sialic acid specific lectin and its biomedical application. Prog Clin Biol Res. 1984;157:31–41. [PubMed] [Google Scholar]
  15. O'Hare K. H. Fine structural observations of ruthenium red binding in developing and adult rat lung. Anat Rec. 1974 Feb;178(2):267–287. doi: 10.1002/ar.1091780210. [DOI] [PubMed] [Google Scholar]
  16. Pinto da Silva P., Torrisi M. R., Kachar B. Freeze-fracture cytochemistry: localization of wheat-germ agglutinin and concanavalin A binding sites on freeze-fractured pancreatic cells. J Cell Biol. 1981 Nov;91(2 Pt 1):361–372. doi: 10.1083/jcb.91.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roth J. Application of lectin--gold complexes for electron microscopic localization of glycoconjugates on thin sections. J Histochem Cytochem. 1983 Aug;31(8):987–999. doi: 10.1177/31.8.6190857. [DOI] [PubMed] [Google Scholar]
  18. Sato A., Spicer S. S. Ultrastructural visualization of galactosyl residues in various alimentary epithelial cells with the peanut lectin-horseradish peroxidase procedure. Histochemistry. 1982;73(4):607–624. doi: 10.1007/BF00493373. [DOI] [PubMed] [Google Scholar]
  19. Suzuki S., Tsuyama S., Suganuma T., Yamamoto N., Murata F. Postembedding staining of Brunner's gland with lectin-ferritin conjugates. J Histochem Cytochem. 1981 Aug;29(8):946–952. doi: 10.1177/29.8.6168680. [DOI] [PubMed] [Google Scholar]
  20. Vasta G. R., Marchalonis J. J. Distribution, specificity and macromolecular properties of tunicate plasma lectins. Prog Clin Biol Res. 1984;157:125–141. [PubMed] [Google Scholar]

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