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. 1986 Jan;5(1):37–40. doi: 10.1002/j.1460-2075.1986.tb04174.x

Molecular characterization of messenger RNAs for `pathogenesis-related' proteins 1a, 1b and 1c, induced by TMV infection of tobacco

Ben J C Cornelissen 1, Rob A M Hooft van Huijsduijnen 1, Leendert C Van Loon 1, John F Bol 1
PMCID: PMC1166692  PMID: 16453665

Abstract

A cDNA library was made to poly(A)-containing RNA from tobacco mosaic virus (TMV)-infected Samsun NN tobacco plants and clones corresponding to mRNAs for the `pathogenesis-related' (PR) proteins 1a, 1b and 1c were identified. One clone was found to contain a complete copy of PR-1b mRNA. The structural organization of this RNA is: a leader sequence of 29 nucleotides, an open reading frame of 504 nucleotides encoding a 30 amino acid long signal peptide and a 138 amino acid long mature protein, and a 3'-non-coding region of 235 nucleotides. Two other clones were found to contain partial copies of PR-1a and PR-1c mRNAs. The data indicate an ∼90% homology between the amino acid sequences of PR-1a, -1b and -1c. Using one of the clones as probe it was shown that in the TMV-inoculated lower leaves and the non-inoculated upper leaves of a tobacco plant, the PR-1 mRNAs become detectable from 2 and 8 days after inoculation, respectively.

Keywords: gene family, pathogenesis-related proteins, TMV infection, tobaccco

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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