. 2024 Nov 13;10(23):e40205. doi: 10.1016/j.heliyon.2024.e40205

Table 1.

Primers used in this study to detect tick-borne pathogens (TBPs) infections in ticks on the Qinghai Lake National Nature Reserve.

Organism	Target gene	Primer sequence (5’∼3’)	Fragment (bp)	Note	References
Tick	ITS2	CGAGACTTGGTGTGAATTGCA	137–1695		[33]
Tick		TCCCATACACCACATTTCCCG	137–1695		[33]
SFG Rickettsia	ompA	GCTTTATTCACCACCTCAAC	209/212		[34]
		TRATCACCACCGTAAGTAAAT	209/212		[34]
	Sca4	CGATGGTAGCATTAAAAGCT	624		[35]
		CTTGCTTTTCAGCAATATCAC	624		[35]
A. phagocytophilum	16S rRNA	CACATGCAAGTCGAACGGATTATTC	932	1st PCR	[36]
		TTCCGTTAAGAAGGATCTAATCTCC	932	1st PCR
		AACGGATTATTCTTTATAGCTTGCT	546/565	Nested PCR
		GGCAGTATTAAAAGCAGCTCCAGG	546/565	Nested PCR
B. microti	18S rRNA	CTTAGTATAAGCTTTTATACAGC	238	1st PCR	[37]
		ATAGGTCAGAAACTTGAATGATACA	238	1st PCR
		GTTATAGTTTATTTGATGTTCGTTT	154	Nested PCR
		AAGCCATGCGATTCGCTAAT	154	Nested PCR
Theileria spp.	18S rRNA	GAAACGGCTACCACATCT	778	1st PCR	[38]
		AGTTTCCCCGTGTTGAGT	778	1st PCR
		TTAAACCTCTTCCAGAGT	581	Nested PCR
		TCAGCCTTGCGACCATAC	581	Nested PCR
B. burgdorferi	23S rRNA	AGAAGTGCTGGAGTCGA	261		[39]
B. burgdorferi		TAGTGCTCTACCTCTATTAA	261		[39]
C. burnetii	htpB	GCGGGTGATGGTACCACAACA	501	1st PCR	[40]
		GGCAATCACCAATAAGGGCCG	501	1st PCR
		TTGCTGGAATGAACCCCA	325	Nested PCR
		TCAAGCTCCGCACTCATG	325	Nested PCR
Brucella spp.	omp22	TGATGGGAGGGACCGACTA	494		[41]
Brucella spp.		TGGTTCTTCAGGTTGTTACGC	494		[41]
F. tularensis	16S rRNA	CAGGCCTAACACATGCAAGTC	1300		[42]
F. tularensis		GGGCGGWGTGTACAAGGC	1300		[42]