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. 1986 Jun;5(6):1377–1381. doi: 10.1002/j.1460-2075.1986.tb04370.x

Synthetic lac operator mediates repression through lac repressor when introduced upstream and downstream from lac promoter.

M Besse, B von Wilcken-Bergmann, B Müller-Hill
PMCID: PMC1166951  PMID: 3015603

Abstract

Plasmids were constructed which carry a synthetic lac operator at various distances from the lac promoter. They were tested in vivo for function in the presence and absence of lac repressor. We found significant repression when the lac operator is situated at the 3' end of the lac I gene or at the 5' end of the lac Z gene. When lac operators are inserted at both sites, we found a greater than 150-fold repression. The complex between lac repressor and DNA carrying these two lac operators is exceedingly stable in vitro suggesting that one tetrameric lac repressor may bind to both lac operators.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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