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. 1986 Jul;5(7):1473–1476. doi: 10.1002/j.1460-2075.1986.tb04384.x

Complete sequence and transcript regulation of a cell adhesion protein from aggregating Dictyostelium cells

A Noegel 1, G Gerisch 1, J Stadler 1, M Westphal 1
PMCID: PMC1166967  PMID: 16453689

Abstract

Three cDNA clones coding for the contact site A (csA) protein, a cell adhesion molecule of Dictyostelium discoideum, were isolated by screening a cDNA library with monoclonal antibodies. Two of these clones contained the complete coding region for the csA protein of 1542 bp including a sequence of 57 bp coding for the leader. The N terminus of the mature protein, as it was published previously, was identified in the amino acid sequence derived from both full-length cDNA clones. Southern blot analysis suggests the presence of only one csA gene in the haploid genome. Accumulation of the csA-specific message of 1.9 kb begins during development on nitrocellulose filters at 9 h of starvation, and reaches a maximum at 12 h, the time of cell aggregation. Expression of the csA glycoprotein follows closely accumulation of the transcripts. In the multicellular slug stage following cell aggregation, the amount of csA transcripts rapidly declines to low levels.

Keywords: cell adhesion protein, contact site A sequence, developmental gene regulation, Dictyostelium

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertholdt G., Stadler J., Bozzaro S., Fichtner B., Gerisch G. Carbohydrate and other epitopes of the contact site A glycoprotein of Dictyostelium discoideum as characterized by monoclonal antibodies. Cell Differ. 1985 May;16(3):187–202. doi: 10.1016/0045-6039(85)90516-0. [DOI] [PubMed] [Google Scholar]
  2. Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. doi: 10.1002/9780470122921.ch2. [DOI] [PubMed] [Google Scholar]
  3. Gerisch G., Hagmann J., Hirth P., Rossier C., Weinhart U., Westphal M. Early Dictyostelium development: control mechanisms bypassed by sequential mutagenesis. Cold Spring Harb Symp Quant Biol. 1985;50:813–822. doi: 10.1101/sqb.1985.050.01.099. [DOI] [PubMed] [Google Scholar]
  4. Gerisch G. Inter-relation of cell adhesion and differentiation in Dictyostelium discoideum. J Cell Sci Suppl. 1986;4:201–219. doi: 10.1242/jcs.1986.supplement_4.13. [DOI] [PubMed] [Google Scholar]
  5. Hohmann H. P., Gerisch G., Lee R. W., Huttner W. B. Cell-free sulfation of the contact site A glycoprotein of Dictyostelium discoideum and of a partially glycosylated precursor. J Biol Chem. 1985 Nov 5;260(25):13869–13878. [PubMed] [Google Scholar]
  6. Marquart M., Deisenhofer J., Huber R., Palm W. Crystallographic refinement and atomic models of the intact immunoglobulin molecule Kol and its antigen-binding fragment at 3.0 A and 1.0 A resolution. J Mol Biol. 1980 Aug 25;141(4):369–391. doi: 10.1016/0022-2836(80)90252-1. [DOI] [PubMed] [Google Scholar]
  7. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  8. Murray B. A., Yee L. D., Loomis W. F. Immunological analysis of glycoprotein (contact sites A) involved in intercellular adhesion of Dictyostelium discoideum. J Supramol Struct Cell Biochem. 1981;17(3):197–211. doi: 10.1002/jsscb.380170302. [DOI] [PubMed] [Google Scholar]
  9. Müller K., Gerisch G. A specific glycoprotein as the target site of adhesion blocking Fab in aggregating Dictyostelium cells. Nature. 1978 Aug 3;274(5670):445–449. doi: 10.1038/274445a0. [DOI] [PubMed] [Google Scholar]
  10. Müller K., Gerisch G., Fromme I., Mayer H., Tsugita A. A membrane glycoprotein of aggregating Dictyostelium cells with the properties of contact sites. Eur J Biochem. 1979 Sep;99(2):419–426. doi: 10.1111/j.1432-1033.1979.tb13271.x. [DOI] [PubMed] [Google Scholar]
  11. Newell P. C., Telser A., Sussman M. Alternative developmental pathways determined by environmental conditions in the cellular slime mold Dictyostelium discoideum. J Bacteriol. 1969 Nov;100(2):763–768. doi: 10.1128/jb.100.2.763-768.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Noegel A., Harloff C., Hirth P., Merkl R., Modersitzki M., Stadler J., Weinhart U., Westphal M., Gerisch G. Probing an adhesion mutant of Dictyostelium discoideum with cDNA clones and monoclonal antibodies indicates a specific defect in the contact site A glycoprotein. EMBO J. 1985 Dec 30;4(13B):3805–3810. doi: 10.1002/j.1460-2075.1985.tb04151.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Noegel A., Welker D. L., Metz B. A., Williams K. L. Presence of nuclear associated plasmids in the lower eukaryote Dictyostelium discoideum. J Mol Biol. 1985 Sep 20;185(2):447–450. doi: 10.1016/0022-2836(85)90416-4. [DOI] [PubMed] [Google Scholar]
  14. Ochiai H., Stadler J., Westphal M., Wagle G., Merkl R., Gerisch G. Monoclonal antibodies against contact sites A of Dictyostelium discoideum: detection of modifications of the glycoprotein in tunicamycin-treated cells. EMBO J. 1982;1(8):1011–1016. doi: 10.1002/j.1460-2075.1982.tb01286.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pagh K., Maruta H., Claviez M., Gerisch G. Localization of two phosphorylation sites adjacent to a region important for polymerization on the tail of Dictyostelium myosin. EMBO J. 1984 Dec 20;3(13):3271–3278. doi: 10.1002/j.1460-2075.1984.tb02289.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Palatnik C. M., Storti R. V., Jacobson A. Fractionation and functional analysis of newly synthesized and decaying messenger RNAs from vegetative cells of Dictyostelium discoideum. J Mol Biol. 1979 Mar 5;128(3):371–395. doi: 10.1016/0022-2836(79)90093-7. [DOI] [PubMed] [Google Scholar]
  17. Putnam F. W., Liu Y. S., Low T. L. Primary structure of a human IgA1 immunoglobulin. IV. Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain. J Biol Chem. 1979 Apr 25;254(8):2865–2874. [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stadler J., Bordier C., Lottspeich F., Henschen A., Gerisch G. Improved purification and N-terminal amino acid sequence determination of the contact site A glycoprotein of Dictyostelium discoideum. Hoppe Seylers Z Physiol Chem. 1982 Aug;363(8):771–776. doi: 10.1515/bchm2.1982.363.2.771. [DOI] [PubMed] [Google Scholar]
  20. Strauss E. C., Kobori J. A., Siu G., Hood L. E. Specific-primer-directed DNA sequencing. Anal Biochem. 1986 Apr;154(1):353–360. doi: 10.1016/0003-2697(86)90536-1. [DOI] [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Watts D. J., Ashworth J. M. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970 Sep;119(2):171–174. doi: 10.1042/bj1190171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Williams J. G., North M. J., Mahbubani H. A developmentally regulated cysteine proteinase in Dictyostelium discoideum. EMBO J. 1985 Apr;4(4):999–1006. doi: 10.1002/j.1460-2075.1985.tb03730.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Young R. A., Davis R. W. Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194–1198. doi: 10.1073/pnas.80.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]

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