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. 1986 Jul;5(7):1667–1673. doi: 10.1002/j.1460-2075.1986.tb04410.x

Heat shock and ecdysterone activation of the Drosophila melanogaster hsp23 gene; a sequence element implied in developmental regulation.

R Mestril, P Schiller, J Amin, H Klapper, J Ananthan, R Voellmy
PMCID: PMC1166993  PMID: 3091364

Abstract

The regulation of the Drosophila melanogaster hsp23 gene by heat shock and ecdysterone has been analysed by measuring activities of hsp--Escherichia coli beta-galactosidase hybrid genes in transfected hormone-sensitive D. melanogaster cells. Mutation analysis identified multiple, distinct promoter elements. A sequence element, which also occurs in the promoters of several other developmentally regulated Drosophila genes, is present in regions of the hsp23 promoter that are essential for its ecdysterone, but not its heat-regulated activity; this element may represent a binding site for an ecdysterone--receptor complex. Mutant promoters that can be activated only by heat shock or by hormone have been constructed. Thus the two types of regulation of the hsp23 gene can function independently of each other.

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Selected References

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  1. Amin J., Mestril R., Lawson R., Klapper H., Voellmy R. The heat shock consensus sequence is not sufficient for hsp70 gene expression in Drosophila melanogaster. Mol Cell Biol. 1985 Jan;5(1):197–203. doi: 10.1128/mcb.5.1.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ayme A., Tissières A. Locus 67B of Drosophila melanogaster contains seven, not four, closely related heat shock genes. EMBO J. 1985 Nov;4(11):2949–2954. doi: 10.1002/j.1460-2075.1985.tb04028.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger E. M., Morganelli C. M. Drosophila cells and ecdysterone: a model system for gene regulation. In Vitro. 1984 Dec;20(12):959–974. doi: 10.1007/BF02619669. [DOI] [PubMed] [Google Scholar]
  4. Dudler R., Travers A. A. Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies. Cell. 1984 Sep;38(2):391–398. doi: 10.1016/0092-8674(84)90494-x. [DOI] [PubMed] [Google Scholar]
  5. Ireland R. C., Berger E., Sirotkin K., Yund M. A., Osterbur D., Fristrom J. Ecdysterone induces the transcription of four heat-shock genes in Drosophila S3 cells and imaginal discs. Dev Biol. 1982 Oct;93(2):498–507. doi: 10.1016/0012-1606(82)90137-3. [DOI] [PubMed] [Google Scholar]
  6. Lawson R., Mestril R., Luo Y., Voellmy R. Ecdysterone selectively stimulates the expression of a 23000-Da heat-shock protein-beta-galactosidase hybrid gene in cultured Drosophila cells. Dev Biol. 1985 Aug;110(2):321–330. doi: 10.1016/0012-1606(85)90091-0. [DOI] [PubMed] [Google Scholar]
  7. Lawson R., Mestril R., Schiller P., Voellmy R. Expression of heat shock-beta-galactosidase hybrid genes in cultured Drosophila cells. Mol Gen Genet. 1984;198(2):116–124. doi: 10.1007/BF00328710. [DOI] [PubMed] [Google Scholar]
  8. Maroy P., Dennis R., Beckers C., Sage B. A., O'Connor J. D. Demonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6035–6038. doi: 10.1073/pnas.75.12.6035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McGinnis W., Shermoen A. W., Heemskerk J., Beckendorf S. K. DNA sequence changes in an upstream DNase I-hypersensitive region are correlated with reduced gene expression. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1063–1067. doi: 10.1073/pnas.80.4.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mellon P., Parker V., Gluzman Y., Maniatis T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system. Cell. 1981 Dec;27(2 Pt 1):279–288. doi: 10.1016/0092-8674(81)90411-6. [DOI] [PubMed] [Google Scholar]
  12. Mestril R., Rungger D., Schiller P., Voellmy R. Identification of a sequence element in the promoter of the Drosophila melanogaster hsp23 gene that is required for its heat activation. EMBO J. 1985 Nov;4(11):2971–2976. doi: 10.1002/j.1460-2075.1985.tb04031.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mirault M. E., Southgate R., Delwart E. Regulation of heat-shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells. EMBO J. 1982;1(10):1279–1285. doi: 10.1002/j.1460-2075.1982.tb00025.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morganelli C. M., Berger E. M., Pelham H. R. Transcription of Drosophila small hsp-tk hybrid genes is induced by heat shock and by ecdysterone in transfected Drosophila cells. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5865–5869. doi: 10.1073/pnas.82.17.5865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Möritz T., Edström J. E., Pongs O. Cloning of a gene localized and expressed at the ecdysteroid regulated puff 74EF in salivary glands of Drosophila larvae. EMBO J. 1984 Feb;3(2):289–295. doi: 10.1002/j.1460-2075.1984.tb01798.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. O'Malley B. W. Studies on the molecular mechanism of steroid hormone action. Harvey Lect. 1978;72:53–90. [PubMed] [Google Scholar]
  17. Parker C. S., Topol J. A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene. Cell. 1984 May;37(1):273–283. doi: 10.1016/0092-8674(84)90323-4. [DOI] [PubMed] [Google Scholar]
  18. Parslow T. G., Blair D. L., Murphy W. J., Granner D. K. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence. Proc Natl Acad Sci U S A. 1984 May;81(9):2650–2654. doi: 10.1073/pnas.81.9.2650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pelham H. R. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene. Cell. 1982 Sep;30(2):517–528. doi: 10.1016/0092-8674(82)90249-5. [DOI] [PubMed] [Google Scholar]
  20. Sage B. A., Tanis M. A., O'Connor J. D. Characterization of ecdysteroid receptors in cytosol and naive nuclear preparations of Drosophila Kc cells. J Biol Chem. 1982 Jun 10;257(11):6373–6379. [PubMed] [Google Scholar]
  21. Savakis C., Demetri G., Cherbas P. Ecdysteroid-inducible polypeptides in a Drosophila cell line. Cell. 1980 Dec;22(3):665–674. doi: 10.1016/0092-8674(80)90542-5. [DOI] [PubMed] [Google Scholar]
  22. Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J Embryol Exp Morphol. 1972 Apr;27(2):353–365. [PubMed] [Google Scholar]
  23. Simon J. A., Sutton C. A., Lobell R. B., Glaser R. L., Lis J. T. Determinants of heat shock-induced chromosome puffing. Cell. 1985 Apr;40(4):805–817. doi: 10.1016/0092-8674(85)90340-x. [DOI] [PubMed] [Google Scholar]
  24. Sirotkin K., Davidson N. Developmentally regulated transcription from Drosophila melanogaster chromosomal site 67B. Dev Biol. 1982 Jan;89(1):196–210. doi: 10.1016/0012-1606(82)90307-4. [DOI] [PubMed] [Google Scholar]
  25. Southgate R., Ayme A., Voellmy R. Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B. J Mol Biol. 1983 Mar 25;165(1):35–57. doi: 10.1016/s0022-2836(83)80241-1. [DOI] [PubMed] [Google Scholar]
  26. Weiher H., König M., Gruss P. Multiple point mutations affecting the simian virus 40 enhancer. Science. 1983 Feb 11;219(4585):626–631. doi: 10.1126/science.6297005. [DOI] [PubMed] [Google Scholar]
  27. Wu C. Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin. Nature. 1984 Sep 6;311(5981):81–84. doi: 10.1038/311081a0. [DOI] [PubMed] [Google Scholar]
  28. Yund M. A., King D. S., Fristrom J. W. Ecdysteroid receptors in imaginal discs of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6039–6043. doi: 10.1073/pnas.75.12.6039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Zimmerman J. L., Petri W., Meselson M. Accumulation of a specific subset of D. melanogaster heat shock mRNAs in normal development without heat shock. Cell. 1983 Apr;32(4):1161–1170. doi: 10.1016/0092-8674(83)90299-4. [DOI] [PubMed] [Google Scholar]
  30. von der Ahe D., Janich S., Scheidereit C., Renkawitz R., Schütz G., Beato M. Glucocorticoid and progesterone receptors bind to the same sites in two hormonally regulated promoters. Nature. 1985 Feb 21;313(6004):706–709. doi: 10.1038/313706a0. [DOI] [PubMed] [Google Scholar]

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