Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1986 Jul;5(7):1697–1703. doi: 10.1002/j.1460-2075.1986.tb04413.x

Two RNA species co-purify with RNase P from the fission yeast Schizosaccharomyces pombe.

G Krupp, B Cherayil, D Frendewey, S Nishikawa, D Söll
PMCID: PMC1166996  PMID: 3743551

Abstract

RNase P activity from Schizosaccharomyces pombe co-purifies with two RNA species. These RNAs are associated with enzyme activity as judged by titrated micrococcal nuclease inactivation experiments. The two RNAs, K1- and K2-RNA, are 285 and 270 nucleotides long, respectively. Both RNAs are transcribed from one gene, present in a single copy in the haploid genome. The primary and a secondary structure of K RNAs have been determined and compared with M1 RNA, their counterpart from Escherichia coli. Very limited sequence homology was observed, and this agrees with the finding that no cross-hybridization with M1 RNA can be detected in a Southern analysis with yeast genomic DNA. However, the secondary structures of K RNA and M1 RNA show the same basic organization and one conserved local motif, the sequence GUG--AGGPu in an exposed hairpin loop.

Full text

PDF
1697

Images in this article

1698Fig. 1.
on p.1698
1698Fig. 2.
on p.1698
1699Fig. 3.
on p.1699
1699Fig. 4.
on p.1699
1700Fig. 7.
on p.1700

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akaboshi E., Guerrier-Takada C., Altman S. Veal heart ribonuclease P has an essential RNA component. Biochem Biophys Res Commun. 1980 Sep 30;96(2):831–837. doi: 10.1016/0006-291x(80)91430-8. [DOI] [PubMed] [Google Scholar]
  2. Baer M., Altman S. A catalytic RNA and its gene from Salmonella typhimurium. Science. 1985 May 24;228(4702):999–1002. doi: 10.1126/science.2408335. [DOI] [PubMed] [Google Scholar]
  3. Branlant C., Krol A., Ebel J. P., Gallinaro H., Lazar E., Jacob M. The conformation of chicken, rat and human U1A RNAs in solution. Nucleic Acids Res. 1981 Feb 25;9(4):841–858. doi: 10.1093/nar/9.4.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brimacombe R. Secondary structure and evolution of ribosomal RNA. Nature. 1981 Nov 19;294(5838):209–210. doi: 10.1038/294209a0. [DOI] [PubMed] [Google Scholar]
  5. Bünemann H., Westhoff P. Hybrid selection of specific RNAs using DNA covalently coupled to macroporous supports. Methods Enzymol. 1983;100:400–407. doi: 10.1016/0076-6879(83)00069-5. [DOI] [PubMed] [Google Scholar]
  6. Chabot B., Black D. L., LeMaster D. M., Steitz J. A. The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein. Science. 1985 Dec 20;230(4732):1344–1349. doi: 10.1126/science.2933810. [DOI] [PubMed] [Google Scholar]
  7. Cooley L., Appel B., Söll D. Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6475–6479. doi: 10.1073/pnas.79.21.6475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dassarma S., Rajbhandary U. L., Khorana H. G. Bacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains. Proc Natl Acad Sci U S A. 1984 Jan;81(1):125–129. doi: 10.1073/pnas.81.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Delihas N., Andersen J. Generalized structures of the 5S ribosomal RNAs. Nucleic Acids Res. 1982 Nov 25;10(22):7323–7344. doi: 10.1093/nar/10.22.7323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Deutscher M. P. Processing of tRNA in prokaryotes and eukaryotes. CRC Crit Rev Biochem. 1984;17(1):45–71. doi: 10.3109/10409238409110269. [DOI] [PubMed] [Google Scholar]
  11. Donis-Keller H. Site specific enzymatic cleavage of RNA. Nucleic Acids Res. 1979 Sep 11;7(1):179–192. doi: 10.1093/nar/7.1.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Efstratiadis A., Vournakis J. N., Donis-Keller H., Chaconas G., Dougall D. K., Kafatos F. C. End labeling of enzymatically decapped mRNA. Nucleic Acids Res. 1977 Dec;4(12):4165–4174. doi: 10.1093/nar/4.12.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Engelke D. R., Gegenheimer P., Abelson J. Nucleolytic processing of a tRNAArg-tRNAAsp dimeric precursor by a homologous component from Saccharomyces cerevisiae. J Biol Chem. 1985 Jan 25;260(2):1271–1279. [PubMed] [Google Scholar]
  14. Garber R. L., Altman S. In vitro processing of B. mori transfer RNA precursor molecules. Cell. 1979 Jun;17(2):389–397. doi: 10.1016/0092-8674(79)90165-x. [DOI] [PubMed] [Google Scholar]
  15. Gardiner K., Pace N. R. RNase P of Bacillus subtilis has a RNA component. J Biol Chem. 1980 Aug 25;255(16):7507–7509. [PubMed] [Google Scholar]
  16. Gold H. A., Altman S. Reconstitution of RNAase P activity using inactive subunits from E. coli and HeLa cells. Cell. 1986 Jan 31;44(2):243–249. doi: 10.1016/0092-8674(86)90758-0. [DOI] [PubMed] [Google Scholar]
  17. Guerrier-Takada C., Altman S. Structure in solution of M1 RNA, the catalytic subunit of ribonuclease P from Escherichia coli. Biochemistry. 1984 Dec 18;23(26):6327–6334. doi: 10.1021/bi00321a006. [DOI] [PubMed] [Google Scholar]
  18. Guerrier-Takada C., Gardiner K., Marsh T., Pace N., Altman S. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell. 1983 Dec;35(3 Pt 2):849–857. doi: 10.1016/0092-8674(83)90117-4. [DOI] [PubMed] [Google Scholar]
  19. Guerrier-Takada C., Haydock K., Allen L., Altman S. Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from Escherichia coli. Biochemistry. 1986 Apr 8;25(7):1509–1515. doi: 10.1021/bi00355a006. [DOI] [PubMed] [Google Scholar]
  20. Gupta R. Halobacterium volcanii tRNAs. Identification of 41 tRNAs covering all amino acids, and the sequences of 33 class I tRNAs. J Biol Chem. 1984 Aug 10;259(15):9461–9471. [PubMed] [Google Scholar]
  21. Hahn S., Hoar E. T., Guarente L. Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8562–8566. doi: 10.1073/pnas.82.24.8562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Heyer W. D., Sipiczki M., Kohli J. Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element. Mol Cell Biol. 1986 Jan;6(1):80–89. doi: 10.1128/mcb.6.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Hindley J., Phear G. A. Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases. Gene. 1984 Nov;31(1-3):129–134. doi: 10.1016/0378-1119(84)90203-8. [DOI] [PubMed] [Google Scholar]
  24. Jacobson A. B., Good L., Simonetti J., Zuker M. Some simple computational methods to improve the folding of large RNAs. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):45–52. doi: 10.1093/nar/12.1part1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Kester W. R., Matthews B. W. Comparison of the structures of carboxypeptidase A and thermolysin. J Biol Chem. 1977 Nov 10;252(21):7704–7710. [PubMed] [Google Scholar]
  26. Klekamp M. S., Weil P. A. Specific transcription of homologous class III genes in yeast-soluble cell-free extracts. J Biol Chem. 1982 Jul 25;257(14):8432–8441. [PubMed] [Google Scholar]
  27. Kline L., Nishikawa S., Söll D. Partial purification of RNase P from Schizosaccharomyces pombe. J Biol Chem. 1981 May 25;256(10):5058–5063. [PubMed] [Google Scholar]
  28. Kole R., Altman S. Reconstitution of RNase P activity from inactive RNA and protein. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3795–3799. doi: 10.1073/pnas.76.8.3795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Krupp G., Thurianx P., Willis I., Gamulin V., Söll D. First identification of an amber nonsense mutation in Schizosaccharomyces pombe: major differences in the efficiency of homologous versus heterologous yeast suppressor tRNA genes. Mol Gen Genet. 1985;201(1):82–87. doi: 10.1007/BF00397990. [DOI] [PubMed] [Google Scholar]
  30. Krämer A., Keller W., Appel B., Lührmann R. The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors. Cell. 1984 Aug;38(1):299–307. doi: 10.1016/0092-8674(84)90551-8. [DOI] [PubMed] [Google Scholar]
  31. Lerner M. R., Steitz J. A. Snurps and scyrps. Cell. 1981 Aug;25(2):298–300. doi: 10.1016/0092-8674(81)90047-7. [DOI] [PubMed] [Google Scholar]
  32. Luehrsen K. R., Nicholson D. E., Eubanks D. C., Fox G. E. An archaebacterial 5S rRNA contains a long insertion sequence. Nature. 1981 Oct 29;293(5835):755–756. doi: 10.1038/293755a0. [DOI] [PubMed] [Google Scholar]
  33. Pedersen N., Hellung-Larsen P., Engberg J. Small nuclear RNAs in the ciliate Tetrahymena. Nucleic Acids Res. 1985 Jun 11;13(11):4203–4224. doi: 10.1093/nar/13.11.4203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Roberts T. M., Swanberg S. L., Poteete A., Riedel G., Backman K. A plasmid cloning vehicle allowing a positive selection for inserted fragments. Gene. 1980 Dec;12(1-2):123–127. doi: 10.1016/0378-1119(80)90022-0. [DOI] [PubMed] [Google Scholar]
  35. Rubin G. M. Preparation of RNA and ribosomes from yeast. Methods Cell Biol. 1975;12:45–64. doi: 10.1016/s0091-679x(08)60951-6. [DOI] [PubMed] [Google Scholar]
  36. Sharp S. J., Schaack J., Cooley L., Burke D. J., Söll D. Structure and transcription of eukaryotic tRNA genes. CRC Crit Rev Biochem. 1985;19(2):107–144. doi: 10.3109/10409238509082541. [DOI] [PubMed] [Google Scholar]
  37. Sprinzl M., Moll J., Meissner F., Hartmann T. Compilation of tRNA sequences. Nucleic Acids Res. 1985;13 (Suppl):r1–49. doi: 10.1093/nar/13.suppl.r1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Sripati C. E., Warner J. R. Isolation, characterization, and translation of mRNA from yeast. Methods Cell Biol. 1978;20:61–81. doi: 10.1016/s0091-679x(08)62009-9. [DOI] [PubMed] [Google Scholar]
  39. Stark B. C., Kole R., Bowman E. J., Altman S. Ribonuclease P: an enzyme with an essential RNA component. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3717–3721. doi: 10.1073/pnas.75.8.3717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Tinoco I., Jr, Uhlenbeck O. C., Levine M. D. Estimation of secondary structure in ribonucleic acids. Nature. 1971 Apr 9;230(5293):362–367. doi: 10.1038/230362a0. [DOI] [PubMed] [Google Scholar]
  41. Udem S. A., Warner J. R. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. doi: 10.1016/0022-2836(72)90279-3. [DOI] [PubMed] [Google Scholar]
  42. Wengler G., Wengler G., Gross H. S. Replicative form of Semliki Forest virus RNA contains an unpaired guanosine. Nature. 1979 Dec 13;282(5740):754–756. doi: 10.1038/282754a0. [DOI] [PubMed] [Google Scholar]
  43. Zagorska L., Van Duin J., Noller H. F., Pace B., Johnson K. D., Pace N. R. The conserved 5 S rRNA complement to tRNA is not required for translation of natural mRNA. J Biol Chem. 1984 Mar 10;259(5):2798–2802. [PubMed] [Google Scholar]
  44. Zoller M. J., Smith M. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res. 1982 Oct 25;10(20):6487–6500. doi: 10.1093/nar/10.20.6487. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES