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. 1986 Sep;5(9):2097–2101. doi: 10.1002/j.1460-2075.1986.tb04471.x

Transport of horseradish peroxidase from the cell surface to the Golgi in insulin-secreting cells: preferential labelling of cisternae located in an intermediate position in the stack.

L Orci, M Ravazzola, M Amherdt, D Brown, A Perrelet
PMCID: PMC1167087  PMID: 3536484

Abstract

We have used serial sectioning to study the topology of Golgi cisternae in insulin-secreting cells during secretion-stimulated endocytotic uptake of exogenous horseradish peroxidase (HRP). HRP-labelled cisternae were followed on several series of consecutive sections. This revealed that labelled cisternae could always be traced to a position in the Golgi stack intermediate between the cis and the trans poles. This occurred in spite of the apparent cis or trans locations of HRP-containing cisternae on some sections. The latter images could be explained by the lack of the true cis or trans (clathrin-coated) cisternae at certain levels of the stack.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Farquhar M. G., Palade G. E. The Golgi apparatus (complex)-(1954-1981)-from artifact to center stage. J Cell Biol. 1981 Dec;91(3 Pt 2):77s–103s. doi: 10.1083/jcb.91.3.77s. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Farquhar M. G. Progress in unraveling pathways of Golgi traffic. Annu Rev Cell Biol. 1985;1:447–488. doi: 10.1146/annurev.cb.01.110185.002311. [DOI] [PubMed] [Google Scholar]
  3. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  4. Griffiths G., Pfeiffer S., Simons K., Matlin K. Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane. J Cell Biol. 1985 Sep;101(3):949–964. doi: 10.1083/jcb.101.3.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Griffiths G., Quinn P., Warren G. Dissection of the Golgi complex. I. Monensin inhibits the transport of viral membrane proteins from medial to trans Golgi cisternae in baby hamster kidney cells infected with Semliki Forest virus. J Cell Biol. 1983 Mar;96(3):835–850. doi: 10.1083/jcb.96.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Herzog V., Farquhar M. G. Luminal membrane retrieved after exocytosis reaches most golgi cisternae in secretory cells. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5073–5077. doi: 10.1073/pnas.74.11.5073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kelly R. B. Pathways of protein secretion in eukaryotes. Science. 1985 Oct 4;230(4721):25–32. doi: 10.1126/science.2994224. [DOI] [PubMed] [Google Scholar]
  8. Lacy P. E., Kostianovsky M. Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes. 1967 Jan;16(1):35–39. doi: 10.2337/diab.16.1.35. [DOI] [PubMed] [Google Scholar]
  9. NOVIKOFF A. B., GOLDFISCHER S. Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Proc Natl Acad Sci U S A. 1961 Jun 15;47:802–810. doi: 10.1073/pnas.47.6.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Orci L. Macro- and micro-domains in the endocrine pancreas. Diabetes. 1982 Jun;31(6 Pt 1):538–565. doi: 10.2337/diab.31.6.538. [DOI] [PubMed] [Google Scholar]
  11. Orci L., Malaisse-Lagae F., Ravazzola M., Amherdt M., Renold A. E. Exocytosis-endocytosis coupling in the pancreatic beta cell. Science. 1973 Aug 10;181(4099):561–562. doi: 10.1126/science.181.4099.561. [DOI] [PubMed] [Google Scholar]
  12. Orci L., Perrelet A., Gorden P. Less-understood aspects of the morphology of insulin secretion and binding. Recent Prog Horm Res. 1978;34:95–121. doi: 10.1016/b978-0-12-571134-0.50007-7. [DOI] [PubMed] [Google Scholar]
  13. Orci L., Ravazzola M., Amherdt M., Louvard D., Perrelet A. Clathrin-immunoreactive sites in the Golgi apparatus are concentrated at the trans pole in polypeptide hormone-secreting cells. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5385–5389. doi: 10.1073/pnas.82.16.5385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pelletier G. Secretion and uptake of peroxidase by rat adenohypophyseal cells. J Ultrastruct Res. 1973 Jun;43(5):445–459. doi: 10.1016/s0022-5320(73)90021-x. [DOI] [PubMed] [Google Scholar]
  15. Sawano F., Ravazzola M., Amherdt M., Perrelet A., Orci L. Horseradish peroxidase uptake and crinophagy in insulin-secreting cells. Exp Cell Res. 1986 May;164(1):174–182. doi: 10.1016/0014-4827(86)90464-7. [DOI] [PubMed] [Google Scholar]

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