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. 1986 Oct;5(10):2649–2656. doi: 10.1002/j.1460-2075.1986.tb04547.x

Engrailed gene expression in Drosophila imaginal discs.

D L Brower
PMCID: PMC1167165  PMID: 3536480

Abstract

Genetic and molecular analyses indicate that the Drosophila engrailed gene is required to distinguish posterior from anterior compartments in each segment of the developing animal. Here, the patterns of engrailed expression in the imaginal discs and ventral ganglion of Drosophila larvae are examined, using an antiserum against the engrailed protein and a novel image processing method to reduce non-specific background. As expected, engrailed expression generally is restricted to cells in the posterior compartment of the discs, and the patterns of expression allow refinements in the fate maps of the discs to be made. More significant is the finding that expression of the gene is highly variable in different regions of posterior compartments, suggesting that engrailed may do more than simply specify 'posteriorness'. In the ventral ganglion engrailed appears to be expressed by a subset of cells, primarily in the posterior regions of each segment. In wing discs from animals that are homozygous for the en1 mutation, the pattern of expression of the gene is altered, as opposed to being simply reduced uniformly in the posterior cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beachy P. A., Helfand S. L., Hogness D. S. Segmental distribution of bithorax complex proteins during Drosophila development. Nature. 1985 Feb 14;313(6003):545–551. doi: 10.1038/313545a0. [DOI] [PubMed] [Google Scholar]
  2. Brower D. L., Lawrence P. A., Wilcox M. Clonal analysis of the undifferentiated wing disk of Drosophila. Dev Biol. 1981 Sep;86(2):448–455. doi: 10.1016/0012-1606(81)90203-7. [DOI] [PubMed] [Google Scholar]
  3. Brower D. L. Posterior-to-anterior transformation in engrailed wing imaginal disks of Drosophila. Nature. 1984 Aug 9;310(5977):496–497. doi: 10.1038/310496a0. [DOI] [PubMed] [Google Scholar]
  4. Carroll S. B., Scott M. P. Localization of the fushi tarazu protein during Drosophila embryogenesis. Cell. 1985 Nov;43(1):47–57. doi: 10.1016/0092-8674(85)90011-x. [DOI] [PubMed] [Google Scholar]
  5. DiNardo S., Kuner J. M., Theis J., O'Farrell P. H. Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein. Cell. 1985 Nov;43(1):59–69. doi: 10.1016/0092-8674(85)90012-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eberlein S., Russell M. A. Effects of deficiencies in the engrailed region of Drosophila melanogaster. Dev Biol. 1983 Nov;100(1):227–237. doi: 10.1016/0012-1606(83)90215-4. [DOI] [PubMed] [Google Scholar]
  7. Garcia-Bellido A., Ripoll P., Morata G. Developmental compartmentalisation of the wing disk of Drosophila. Nat New Biol. 1973 Oct 24;245(147):251–253. doi: 10.1038/newbio245251a0. [DOI] [PubMed] [Google Scholar]
  8. Garcia-Bellido A., Santamaria P. Developmental analysis of the wing disc in the mutant engrailed of Drosophila melanogaster. Genetics. 1972 Sep;72(1):87–104. doi: 10.1093/genetics/72.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gergen J. P., Wieschaus E. Dosage requirements for runt in the segmentation of Drosophila embryos. Cell. 1986 Apr 25;45(2):289–299. doi: 10.1016/0092-8674(86)90393-4. [DOI] [PubMed] [Google Scholar]
  10. Karr T. L., Ali Z., Drees B., Kornberg T. The engrailed locus of D. melanogaster provides an essential zygotic function in precellular embryos. Cell. 1985 Dec;43(3 Pt 2):591–601. doi: 10.1016/0092-8674(85)90231-4. [DOI] [PubMed] [Google Scholar]
  11. Kornberg T. Engrailed: a gene controlling compartment and segment formation in Drosophila. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1095–1099. doi: 10.1073/pnas.78.2.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kornberg T., Sidén I., O'Farrell P., Simon M. The engrailed locus of Drosophila: in situ localization of transcripts reveals compartment-specific expression. Cell. 1985 Jan;40(1):45–53. doi: 10.1016/0092-8674(85)90307-1. [DOI] [PubMed] [Google Scholar]
  13. Kuner J. M., Nakanishi M., Ali Z., Drees B., Gustavson E., Theis J., Kauvar L., Kornberg T., O'Farrell P. H. Molecular cloning of engrailed: a gene involved in the development of pattern in Drosophila melanogaster. Cell. 1985 Aug;42(1):309–316. doi: 10.1016/s0092-8674(85)80126-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lawrence P. A., Johnston P. On the role of the engrailed+ gene in the internal organs of Drosophila. EMBO J. 1984 Dec 1;3(12):2839–2844. doi: 10.1002/j.1460-2075.1984.tb02217.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lawrence P. A., Morata G. Compartments in the wing of Drosophila: a study of the engrailed gene. Dev Biol. 1976 Jun;50(2):321–337. doi: 10.1016/0012-1606(76)90155-x. [DOI] [PubMed] [Google Scholar]
  16. Lawrence P. A., Struhl G. Further studies of the engrailed phenotype in Drosophila. EMBO J. 1982;1(7):827–833. doi: 10.1002/j.1460-2075.1982.tb01255.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lewis E. B. A gene complex controlling segmentation in Drosophila. Nature. 1978 Dec 7;276(5688):565–570. doi: 10.1038/276565a0. [DOI] [PubMed] [Google Scholar]
  18. Martinez-Arias A., Lawrence P. A. Parasegments and compartments in the Drosophila embryo. Nature. 1985 Feb 21;313(6004):639–642. doi: 10.1038/313639a0. [DOI] [PubMed] [Google Scholar]
  19. Morata G., Lawrence P. A. Control of compartment development by the engrailed gene in Drosophila. Nature. 1975 Jun 19;255(5510):614–617. doi: 10.1038/255614a0. [DOI] [PubMed] [Google Scholar]
  20. Morata G., Lawrence P. A. Development of the eye-antenna imaginal disc of Drosophila. Dev Biol. 1979 Jun;70(2):355–371. doi: 10.1016/0012-1606(79)90033-2. [DOI] [PubMed] [Google Scholar]
  21. Nüsslein-Volhard C., Wieschaus E. Mutations affecting segment number and polarity in Drosophila. Nature. 1980 Oct 30;287(5785):795–801. doi: 10.1038/287795a0. [DOI] [PubMed] [Google Scholar]
  22. Parkhurst S. M., Corces V. G. Forked, gypsys, and suppressors in Drosophila. Cell. 1985 Jun;41(2):429–437. doi: 10.1016/s0092-8674(85)80016-7. [DOI] [PubMed] [Google Scholar]
  23. Ready D. F., Hanson T. E., Benzer S. Development of the Drosophila retina, a neurocrystalline lattice. Dev Biol. 1976 Oct 15;53(2):217–240. doi: 10.1016/0012-1606(76)90225-6. [DOI] [PubMed] [Google Scholar]
  24. Weir M. P., Kornberg T. Patterns of engrailed and fushi tarazu transcripts reveal novel intermediate stages in Drosophila segmentation. Nature. 1985 Dec 5;318(6045):433–439. doi: 10.1038/318433a0. [DOI] [PubMed] [Google Scholar]
  25. White R. A., Wilcox M. Distribution of Ultrabithorax proteins in Drosophila. EMBO J. 1985 Aug;4(8):2035–2043. doi: 10.1002/j.1460-2075.1985.tb03889.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. White R. A., Wilcox M. Protein products of the bithorax complex in Drosophila. Cell. 1984 Nov;39(1):163–171. doi: 10.1016/0092-8674(84)90202-2. [DOI] [PubMed] [Google Scholar]

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