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. 1986 Dec 20;5(13):3579–3582. doi: 10.1002/j.1460-2075.1986.tb04685.x

The regulation of male-specific transcripts by sex determining genes in Drosophila melanogaster

U Schäfer 1
PMCID: PMC1167396  PMID: 16453743

Abstract

The accumulation of male-specific transcripts in various genotypes of Drosophila melanogaster was analysed by Northern blot hybridization. The genotypes were either hetero- or homozygous for a mutation in one of the genes for somatic sex determination. The aim was to study the role of these genes in regulating male-specific transcription in soma or germ line. All intersexual phenotypes and pseudomales irrespective of their genotypic sex accumulate the male-specific somatic RNA demonstrating that it is regulated by these genes. In contrast to this, the transcript from the male germ line could only be detected in those mutant genotypes that have the male sex chromosome constitution of X/Y, although its synthesis is not dependent on the presence of a Y chromosome. It is, therefore, not under the control of the sex determining loci but directly regulated by the primary signal for sex determination, namely the ratio of X chromosomes to autosomes.

Keywords: Drosophila, male-specific RNAs, sex determining genes, soma, germ line

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker B. S., Belote J. M. Sex determination and dosage compensation in Drosophila melanogaster. Annu Rev Genet. 1983;17:345–393. doi: 10.1146/annurev.ge.17.120183.002021. [DOI] [PubMed] [Google Scholar]
  2. Baker B. S., Ridge K. A. Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster. Genetics. 1980 Feb;94(2):383–423. doi: 10.1093/genetics/94.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barnett T., Pachl C., Gergen J. P., Wensink P. C. The isolation and characterization of Drosophila yolk protein genes. Cell. 1980 Oct;21(3):729–738. doi: 10.1016/0092-8674(80)90436-5. [DOI] [PubMed] [Google Scholar]
  4. Belote J. M., Baker B. S. Sex determination in Drosophila melanogaster: analysis of transformer-2, a sex-transforming locus. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1568–1572. doi: 10.1073/pnas.79.5.1568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Belote J. M., Handler A. M., Wolfner M. F., Livak K. J., Baker B. S. Sex-specific regulation of yolk protein gene expression in Drosophila. Cell. 1985 Feb;40(2):339–348. doi: 10.1016/0092-8674(85)90148-5. [DOI] [PubMed] [Google Scholar]
  6. Bownes M., Nöthiger R. Sex determining genes and vitellogenin synthesis in Drosophila melanogaster. Mol Gen Genet. 1981;182(2):222–228. doi: 10.1007/BF00269661. [DOI] [PubMed] [Google Scholar]
  7. CHAN FUNG S. T., GOWEN J. W. The developmental effect of a sex-limited gene in Drosophila melanogaster. J Exp Zool. 1957 Apr;134(3):515–532. doi: 10.1002/jez.1401340306. [DOI] [PubMed] [Google Scholar]
  8. Fujihara T., Kawabe M., Oishi K. A sex-transformation gene in Drosophila melanogaster. J Hered. 1978 Jul-Aug;69(4):229–236. doi: 10.1093/oxfordjournals.jhered.a108936. [DOI] [PubMed] [Google Scholar]
  9. HILDRETH P. E. DOUBLESEX, RECESSIVE GENE THAT TRANSFORMS BOTH MALES AND FEMALES OF DROSOPHILA INTO INTERSEXES. Genetics. 1965 Apr;51:659–678. doi: 10.1093/genetics/51.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hennig W. Y chromosome function and spermatogenesis in Drosophila hydei. Adv Genet. 1985;23:179–234. doi: 10.1016/s0065-2660(08)60513-1. [DOI] [PubMed] [Google Scholar]
  11. Hess O., Meyer G. F. Genetic activities of the Y chromosome in Drosophila during spermatogenesis. Adv Genet. 1968;14:171–223. doi: 10.1016/s0065-2660(08)60427-7. [DOI] [PubMed] [Google Scholar]
  12. Marsh J. L., Wieschaus E. Is sex determination in germ line and soma controlled by separate genetic mechanisms? Nature. 1978 Mar 16;272(5650):249–251. doi: 10.1038/272249a0. [DOI] [PubMed] [Google Scholar]
  13. Ota T., Fukunaga A., Kawabe M., Oishi K. Interactions between sex-transformation mutants of Drosophila melanogaster. I. Hemolymph vitellogenins and gonad morphology. Genetics. 1981 Nov-Dec;99(3-4):429–441. doi: 10.1093/genetics/99.3-4.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schüpbach T. Autosomal mutations that interfere with sex determination in somatic cells of Drosophila have no direct effect on the germline. Dev Biol. 1982 Jan;89(1):117–127. doi: 10.1016/0012-1606(82)90300-1. [DOI] [PubMed] [Google Scholar]
  15. Schüpbach T. Normal female germ cell differentiation requires the female X chromosome to autosome ratio and expression of sex-lethal in Drosophila melanogaster. Genetics. 1985 Mar;109(3):529–548. doi: 10.1093/genetics/109.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sturtevant A H. A Gene in Drosophila Melanogaster That Transforms Females into Males. Genetics. 1945 May;30(3):297–299. doi: 10.1093/genetics/30.3.297. [DOI] [PMC free article] [PubMed] [Google Scholar]

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