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. 2024 Dec 18;12(12):2625. doi: 10.3390/microorganisms12122625

Figure 3.

Figure 3

Overview of PCR and sequencing strategies for cyanobacterial 16S rRNA genes. The position of the primers used for amplification and sequencing of the 16S rRNA gene and the primary and secondary PCR products are shown. The general structure of the ribosomal rDNA operon with the 16S rRNA gene, two tRNA genes, and 23S rDNA gene is depicted schematically at the top of the figure. The primer sequences are shown in Table 2. Abbreviations used for the primers in the figure refer to the following primer designations and sequences in Table 2: 16S_SG1_short_forw (SG1), 16S H4_forw (H4-forw), ptLSU C-D_rev (PtL CD R), SG2_rev (SG2), Seq_16S_H4_forw (SeqH4), Seq_16S_1040_rev (1040R), Seq_16S_pos874_forw (874F), and Seq_16S_49_rev (Seq H49R).