Abstract
A periodic acid-Schiff-positive granular cell (PAS-G cell) was investigated by light and electron microscopy in the gills of the gilthead seabream (Sparus aurata L.). Despite the use of several fixatives, it was only possible to demonstrate metachromasia after fixing these cells with a solution of 10% formol and 5% acetic acid in methanol. Using this fixative, their granules stained purple with toluidine blue or thionin. In semithin resin sections, the granules also stained purple with toluidine blue-borax. Ultrastructurally, these cells possessed 2 types of homogeneous electron-dense granules and characteristic long thin surface processes resembling a string of beads. These PAS-G cell share morphological features with basophils and mast cells, and were metachromatic. They were observed in sites similar to those for mast cells in mammals.
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- Barber D. L., Westermann J. E. Morphological and histochemical studies on a PAS-positive granular leukocyte in blood and connective tissues of Catostomus commersonii Lacépède (teleostei:pisces). Am J Anat. 1975 Feb;142(2):205–220. doi: 10.1002/aja.1001420206. [DOI] [PubMed] [Google Scholar]
- Galli S. J., Dvorak A. M., Dvorak H. F. Basophils and mast cells: morphologic insights into their biology, secretory patterns, and function. Prog Allergy. 1984;34:1–141. [PubMed] [Google Scholar]
- Lester R. J., Desser S. S. Ultrastructural observations on the granulocytic leucocytes of the teleost Catostomus commersoni. Can J Zool. 1975 Nov;53(11):1648–1657. doi: 10.1139/z75-198. [DOI] [PubMed] [Google Scholar]
- López-Ruiz A., Angeles Esteban M., Meseguer J. Blood cells of the gilthead seabream (Sparus aurata L.): light and electron microscopic studies. Anat Rec. 1992 Oct;234(2):161–171. doi: 10.1002/ar.1092340203. [DOI] [PubMed] [Google Scholar]
- Meseguer J., Esteban M. A., Garcia Ayala A., Lopez Ruiz A., Agulleiro B. Granulopoiesis in the head-kidney of the sea bass (Dicentrarchus labrax L.): an ultrastructural study. Arch Histol Cytol. 1990 Jul;53(3):287–296. doi: 10.1679/aohc.53.287. [DOI] [PubMed] [Google Scholar]
- Powell M. D., Wright G. M., Burka J. F. Degranulation of eosinophilic granule cells induced by capsaicin and substance P in the intestine of the rainbow trout (Oncorhynchus mykiss Walbaum). Cell Tissue Res. 1991 Dec;266(3):469–474. doi: 10.1007/BF00318587. [DOI] [PubMed] [Google Scholar]
- Vallejo A. N., Jr, Ellis A. E. Ultrastructural study of the response of eosinophil granule cells to Aeromonas salmonicida extracellular products and histamine liberators in rainbow trout Salmo gairdneri Richardson. Dev Comp Immunol. 1989 Spring;13(2):133–148. doi: 10.1016/0145-305x(89)90028-1. [DOI] [PubMed] [Google Scholar]
- WEINREB E. L. STUDIES ON THE FINE STRUCTURE OF TELEOST BLOOD CELLS. I. PERIPHERALBLOOD. Anat Rec. 1963 Oct;147:219–238. doi: 10.1002/ar.1091470206. [DOI] [PubMed] [Google Scholar]