Figure 6.

Persistence of BMYV-SUL-induced VIGS in sugar beets at 14 weeks post-infiltration. (a) Young leaves of symptomatic BMYV-SUL-infected beets of line B (left) and line E (right). The red arrows indicate the faint symptoms present on older non-inoculated leaves of line B. (b) Analysis of the infection status of agroinoculated plants of both lines by RT-PCR, using oligonucleotides flanking the inserted SUL fragment site. Bands of 402 bp and 472 bp were respectively expected for wild-type BMYV and recombinant BMYV-SUL constructs. Plasmid DNA of the wild-type and recombinant BMYV constructs were used as PCR size controls. Asymptomatic and symptomatic plants are respectively annotated by a minus (−) or a plus (+) sign.