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. 1974 Jul;141(1):133–139. doi: 10.1042/bj1410133

The cell-envelope glycolipids of baker's yeast

K Työrinoja 1, T Nurminen 1, H Suomalainen 1
PMCID: PMC1168058  PMID: 4616681

Abstract

Sphingolipids were found to dominate in the glycolipids from the cell envelope of baker's yeast. A relatively large quantity of ceramides was detected. Among the several complex phosphosphingolipids described, ceramide-(P-inositol)2-mannose was the main component. About 55% of long-chain bases in sphingolipids consisted of C18-phytosphingosine (4D-hydroxysphinganine). Other bases, found in decreasing concentrations, were C20-phytosphingosine, C20-dehydrophytosphingosine, C18-dihydrosphingosine (sphinganine) and C19-dihydrosphingosine. The presence of sterol glycosides, sulpholipids, cerebrosides and acylglucoses was demonstrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOCH K., GAUTSCHI F., JOHNSTON J. D. Isolation of lanosterol from isocholesterol. J Biol Chem. 1957 Jan;224(1):185–190. [PubMed] [Google Scholar]
  2. Baraud J., Maurice A., Napias C. Composition et répartition des lipides au sein des cellules de Saccharomyces cerevisiae. Bull Soc Chim Biol (Paris) 1970;52(4):421–432. [PubMed] [Google Scholar]
  3. Brady R. N., Di Mari S. J., Snell E. E. Biosynthesis of sphingolipid bases. 3. Isolation and characterization of ketonic intermediates in the synthesis of sphingosine and dihydrosphingosine by cell-free extracts of Hansenula ciferri. J Biol Chem. 1969 Jan 25;244(2):491–496. [PubMed] [Google Scholar]
  4. Brennan P. J., Flynn M. P., Griffin P. F.S. Acylglucoses in Escherichia coli, Saccharomyces cerevisiae and Agaricus bisporus. FEBS Lett. 1970 Jul 3;8(6):322–324. doi: 10.1016/0014-5793(90)80004-3. [DOI] [PubMed] [Google Scholar]
  5. Carter H. E., Gaver R. C. Improved reagent for trimethylsilylation of sphingolipid bases. J Lipid Res. 1967 Jul;8(4):391–395. [PubMed] [Google Scholar]
  6. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  7. Di Mari S. J., Brady R. N., Snell E. E. Biosynthesis of sphingolipid bases. IV. The biosynthetic origin of sphingosine in Hansenula ciferri. Arch Biochem Biophys. 1971 Apr;143(2):553–565. doi: 10.1016/0003-9861(71)90240-2. [DOI] [PubMed] [Google Scholar]
  8. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  9. GAVER R. C., SWEELEY C. C. METHODS FOR METHANOLYSIS OF SPHINGOLIPIDS AND DIRECT DETERMINATION OF LONG-CHAIN BASES BY GAS CHROMATOGRAPHY. J Am Oil Chem Soc. 1965 Apr;42:294–298. doi: 10.1007/BF02540132. [DOI] [PubMed] [Google Scholar]
  10. Heftmann E., Ko S. T., Bennett R. D. Response of steroids to sulfuric acid in thin-layer chromatography. J Chromatogr. 1966 Mar;21(3):490–494. doi: 10.1016/s0021-9673(01)91347-0. [DOI] [PubMed] [Google Scholar]
  11. Hoshi M., Kishimoto Y., Hignite C. 2,3-Erythro-dihydroxyhexacosanoic acid and homologs: isolation from yeast cerebrin phosphate and determination of their structures. J Lipid Res. 1973 Jul;14(4):406–414. [PubMed] [Google Scholar]
  12. Kean E. L. Rapid, sensitive spectrophotometric method for quantitative determination of sulfatides. J Lipid Res. 1968 May;9(3):319–327. [PubMed] [Google Scholar]
  13. Kim J. H., Shome B., Liao T. H., Pierce J. G. Analysis of neutral sugars by gas-liquid chromatography of alditol acetates: application to thyrotropic hormone and other glycoproteins. Anal Biochem. 1967 Aug;20(2):258–274. doi: 10.1016/0003-2697(67)90031-0. [DOI] [PubMed] [Google Scholar]
  14. Kwiterovich P. O., Jr, Sloan H. R., Fredrickson D. S. Glycolipids and other lipid constituents of normal human liver. J Lipid Res. 1970 Jul;11(4):322–330. [PubMed] [Google Scholar]
  15. Lehnhardt W. F., Winzler R. J. Determination of neutral sugars in glycoproteins by gas-liquid chromatography. J Chromatogr. 1968 May 7;34(4):471–479. doi: 10.1016/0021-9673(68)80091-3. [DOI] [PubMed] [Google Scholar]
  16. Lopes J., Inniss W. E. Chemical composition of lipopolysaccharide from an avian strain of Escherichia coli O18. Can J Microbiol. 1970 Nov;16(11):1117–1121. doi: 10.1139/m70-187. [DOI] [PubMed] [Google Scholar]
  17. Nurminen T., Oura E., Suomalainen H. The enzymic composition of the isolated cell wall and plasma membrane of baker's yeast. Biochem J. 1970 Jan;116(1):61–69. doi: 10.1042/bj1160061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nurminen T., Suomalainen H. Occurrence of long-chain fatty acids and glycolipids in the cell envelope fractions of baker's yeast. Biochem J. 1971 Dec;125(4):963–969. doi: 10.1042/bj1250963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pepper D. S., Jamieson G. A. Studies on glycoproteins. 3. Isolation of sialylglycopeptides from human platelet membranes. Biochemistry. 1969 Aug;8(8):3362–3369. doi: 10.1021/bi00836a034. [DOI] [PubMed] [Google Scholar]
  20. Renkonen O. Mono- and dimethyl phosphatidates from different subtypes of choline and ethanolamine glycerophosphatides. Biochim Biophys Acta. 1968 Jan 10;152(1):114–135. doi: 10.1016/0005-2760(68)90014-3. [DOI] [PubMed] [Google Scholar]
  21. Rouser G., Kritchevsky G., Simon G., Nelson G. J. Quantitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids. Lipids. 1967 Jan;2(1):37–40. doi: 10.1007/BF02531998. [DOI] [PubMed] [Google Scholar]
  22. Steiner S., Lester R. L. Studies on the diversity of inositol-containing yeast phospholipids: incorporation of 2-deoxyglucose into lipid. J Bacteriol. 1972 Jan;109(1):81–88. doi: 10.1128/jb.109.1.81-88.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Steiner S., Smith S., Waechter C. J., Lester R. L. Isolation and partial characterization of a major inositol-containing lipid in baker's yeast, mannosyl-diinositol, diphosphoryl-ceramide. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1042–1048. doi: 10.1073/pnas.64.3.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stoffel W. Sphingosine metabolism and its link to phospholipid biosynthesis. Mol Cell Biochem. 1973 Jun 27;1(2):147–155. doi: 10.1007/BF01659326. [DOI] [PubMed] [Google Scholar]
  25. Vance D. E., Sweeley C. C. Quantitative determination of the neutral glycosyl ceramides in human blood. J Lipid Res. 1967 Nov;8(6):621–630. [PubMed] [Google Scholar]
  26. Weinert M., Kljaić K., Prostenik M. Studies in the sphingolipids series. XXXV. Separation and chemical characterization of the cerebrins from the yeast Saccharomyces cerevisiae. Chem Phys Lipids. 1973 Sep;11(2):83–88. doi: 10.1016/0009-3084(73)90025-x. [DOI] [PubMed] [Google Scholar]
  27. Zahler W. L., Barden R. E., Cleland W. W. Some physical properties of palmityl-coenzyme A micelles. Biochim Biophys Acta. 1968 Sep 2;164(1):1–11. doi: 10.1016/0005-2760(68)90065-9. [DOI] [PubMed] [Google Scholar]

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