Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1974 Jul;141(1):317–320. doi: 10.1042/bj1410317

Action of dimeric hybrids of native and selectively alkylated ribonuclease A on double-stranded ribonucleic acid (Short Communication)

Giuseppe D'Alessio 1, Jiří Doskočil 1, Massimo Libonati 1
PMCID: PMC1168081  PMID: 4616683

Abstract

The hypothesis that both active sites of dimeric ribonuclease A have to be simultaneously available in order to make the enzyme able to degrade double-stranded RNA is tested with purified hybrid dimers of ribonuclease A and its carboxymethylated derivative. The results obtained support the hypothesis.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnard E. A. Ribonucleases. Annu Rev Biochem. 1969;38:677–732. doi: 10.1146/annurev.bi.38.070169.003333. [DOI] [PubMed] [Google Scholar]
  2. CRESTFIELD A. M., STEIN W. H., MOORE S. On the aggregation of bovine pancreatic ribonuclease. Arch Biochem Biophys. 1962 Sep;Suppl 1:217–222. [PubMed] [Google Scholar]
  3. CRESTFIELD A. M., STEIN W. H., MOORE S. Properties and conformation of the histidine residues at the active site of ribonuclease. J Biol Chem. 1963 Jul;238:2421–2428. [PubMed] [Google Scholar]
  4. Crestfield A. M., Fruchter R. G. The homologous and hybrid dimers of ribonuclease A and its carboxymethylhistidine derivatives. J Biol Chem. 1967 Jul 25;242(14):3279–3284. [PubMed] [Google Scholar]
  5. D'Alessio G., Floridi A., De Prisco R., Pignero A., Leone E. [Bull semen ribonucleases. 1. Purification and physico-chemical properties of the major component]. Eur J Biochem. 1972 Mar 27;26(2):153–161. doi: 10.1111/j.1432-1033.1972.tb01751.x. [DOI] [PubMed] [Google Scholar]
  6. D'Alessio G., Parente A., Guida C., Leone E. Dimeric structure of seminal ribonuclease. FEBS Lett. 1972 Nov 1;27(2):285–288. doi: 10.1016/0014-5793(72)80642-2. [DOI] [PubMed] [Google Scholar]
  7. D'Alessio G., Zofra S., Libonati M. Action of dimeric ribonucleases on double-stranded RNA. FEBS Lett. 1972 Aug 15;24(3):355–358. doi: 10.1016/0014-5793(72)80390-9. [DOI] [PubMed] [Google Scholar]
  8. Floridi A., D'Alessio G., Leone E. Bull semen ribonucleases. 2. Catalytic properties of the major component. Eur J Biochem. 1972 Mar 27;26(2):162–167. doi: 10.1111/j.1432-1033.1972.tb01752.x. [DOI] [PubMed] [Google Scholar]
  9. Fruchter R. G., Crestfield A. M. On the structure of ribonuclease dimer. Isolation and identification of monomers derived from inactive carboxymethyl dimers. J Biol Chem. 1965 Oct;240(10):3875–3882. [PubMed] [Google Scholar]
  10. Fruchter R. G., Crestfield A. M. Preparation and properties of two active forms of ribonuclease dimer. J Biol Chem. 1965 Oct;240(10):3868–3874. [PubMed] [Google Scholar]
  11. Goren H. J., Barnard E. A. Relation of reactivity to structure in pancreatic ribonuclease. I. An analysis of the various reactions with bromoacetate in the pH range 2-7. Biochemistry. 1970 Feb 17;9(4):959–973. doi: 10.1021/bi00806a036. [DOI] [PubMed] [Google Scholar]
  12. Goren H. J., Barnard E. A. Relations of reactivity to structure in pancreatic ribonuclease. II. Positions of residues alkylated in certain conditions by bromoacetate. Biochemistry. 1970 Feb 17;9(4):974–983. doi: 10.1021/bi00806a037. [DOI] [PubMed] [Google Scholar]
  13. Hausen P., Stein H. Ribonuclease H. An enzyme degrading the RNA moiety of DNA-RNA hybrids. Eur J Biochem. 1970 Jun;14(2):278–283. doi: 10.1111/j.1432-1033.1970.tb00287.x. [DOI] [PubMed] [Google Scholar]
  14. Libonati M. Degradation of poly A and double-stranded RNA by aggregates of pancreatic ribonuclease. Biochim Biophys Acta. 1971 Jan 28;228(2):440–445. doi: 10.1016/0005-2787(71)90049-9. [DOI] [PubMed] [Google Scholar]
  15. Libonati M., Floridi A. Breakdown of double-stranded RNA by bull semen ribonuclease. Eur J Biochem. 1969 Mar;8(1):81–87. doi: 10.1111/j.1432-1033.1969.tb00498.x. [DOI] [PubMed] [Google Scholar]
  16. Lodish H. F., Zinder N. D. Mutants of the bacteriophage f2. 8. Control mechanisms for phage-specific syntheses. J Mol Biol. 1966 Aug;19(2):333–348. doi: 10.1016/s0022-2836(66)80008-6. [DOI] [PubMed] [Google Scholar]
  17. Paces V., Doskocil J., Sorm F. Incorporation of 5-azacytidine into nucleic acids of Escherichia coli. Biochim Biophys Acta. 1968 Jul 23;161(2):352–360. [PubMed] [Google Scholar]
  18. Palese P., Koch G. Degradation of single- and double-stranded RNA by frog virus 3. Proc Natl Acad Sci U S A. 1972 Mar;69(3):698–701. doi: 10.1073/pnas.69.3.698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Robertson H. D., Webster R. E., Zinder N. D. Purification and properties of ribonuclease III from Escherichia coli. J Biol Chem. 1968 Jan 10;243(1):82–91. [PubMed] [Google Scholar]
  20. Stern R. A nuclease from animal serum which hydrolyzes double-stranded RNA. Biochem Biophys Res Commun. 1970 Nov 9;41(3):608–614. doi: 10.1016/0006-291x(70)90056-2. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES