Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1974 Aug;141(2):567–572. doi: 10.1042/bj1410567

Salt-soluble elastin from lathyritic chicks

Bryan C Sykes 1,*, S Miles Partridge 1
PMCID: PMC1168111  PMID: 4455221

Abstract

The isolation of a salt-soluble homogeneous elastin from the aortas of lathyritic chicks by chromatography on DEAE-cellulose and salt precipitation is described. These new techniques, as well as some previously published by other workers, were evaluated with the help of antiserum raised in sheep against insoluble chick elastin. The purified elastin was very basic and behaved in a predictable manner in coacervation studies. The protein migrated in sodium dodecyl sulphate–polyacrylamide gels as a single band moving slightly faster than pyruvate kinase (mol.wt. 57000).

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Etherington D. J. The nature of the collagenolytic cathepsin of rat liver and its distribution in other rat tissues. Biochem J. 1972 May;127(4):685–692. doi: 10.1042/bj1270685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FAHEY J. L., MCKELVEY E. M. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES. J Immunol. 1965 Jan;94:84–90. [PubMed] [Google Scholar]
  3. LEWIS M. S., PIEZ K. A. SEDIMENTATION-EQUILIBRIUM STUDIES OF THE MOLECULAR WEIGHT OF SINGLE AND DOUBLE CHAINS FROM RAT-SKIN COLLAGEN. Biochemistry. 1964 Aug;3:1126–1131. doi: 10.1021/bi00896a020. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Miller E. J. Isolation and characterization of a collagen from chick cartilage containing three identical alpha chains. Biochemistry. 1971 Apr 27;10(9):1652–1659. doi: 10.1021/bi00785a024. [DOI] [PubMed] [Google Scholar]
  6. PARTRIDGE S. M., DAVIS H. F., ADAIR G. S. The chemistry of connective tissues. 2. Soluble proteins derived from partial hydrolysis of elastin. Biochem J. 1955 Sep;61(1):11–21. doi: 10.1042/bj0610011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pinnell S. R., Martin G. R. The cross-linking of collagen and elastin: enzymatic conversion of lysine in peptide linkage to alpha-aminoadipic-delta-semialdehyde (allysine) by an extract from bone. Proc Natl Acad Sci U S A. 1968 Oct;61(2):708–716. doi: 10.1073/pnas.61.2.708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rucker R. B., Goettlich-Riemann W. Isolation and properties of soluble elastin from copper-deficient chicks. J Nutr. 1972 Apr;102(4):563–570. doi: 10.1093/jn/102.4.563. [DOI] [PubMed] [Google Scholar]
  10. Smith D. W., Brown D. M., Carnes W. H. Preparation and properties of salt-soluble elastin. J Biol Chem. 1972 Apr 25;247(8):2427–2432. [PubMed] [Google Scholar]
  11. Smith D. W., Weissman N., Carnes W. H. Cardiovascular studies on copper deficient swine. XII. Partial purification of a soluble protein resembling elastin. Biochem Biophys Res Commun. 1968 May 10;31(3):309–315. doi: 10.1016/0006-291x(68)90476-2. [DOI] [PubMed] [Google Scholar]
  12. Sykes B. C., Bailey A. J. Molecular weight heterogeneity of the alpha-chain sub-units of collagen. Biochem Biophys Res Commun. 1971 Apr 16;43(2):340–345. doi: 10.1016/0006-291x(71)90758-3. [DOI] [PubMed] [Google Scholar]
  13. Sykes B. C., Partridge S. M. Isolation of a soluble elastin from lathyritic chicks. Biochem J. 1972 Dec;130(4):1171–1172. doi: 10.1042/bj1301171. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES