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. 1974 Sep;141(3):617–625. doi: 10.1042/bj1410617

Studies on the 3′-terminal sequences of the large ribosomal ribonucleic acid of different eukaryotes and those associated with `hidden' breaks in heat-dissociable insect 26S ribonucleic acid

John Shine 1, John A Hunt 1,*, Lynn Dalgarno 1
PMCID: PMC1168165  PMID: 4219141

Abstract

The 3′-terminal sequences associated with the large rRNA complex from a range of eukaryotes were determined after pancreatic or T1-ribonuclease digestion of RNA terminally labelled with [3H]isoniazid. In all higher eukaryotes examined except Drosophila melanogaster, the 3′-terminal sequences Y-G-UOH and G-C-UOH were demonstrated for the large RNA component(s) and for 6S RNA respectively. The 3′-terminal sequence of Saccharomyces cerevisiae 26S RNA was Y-G-UOH and that of 6S RNA Y-A-U-U-UOH. Three 3′-terminal sequences were found in equimolar amounts in the heat-dissociable 26S rRNA characteristic of insect ribosomes. These were Y-G-U-G-UOH, Y-C-G-UOH and G-C-UOH for cultured Antheraea eucalypti cells, Y-G-UOH, Y-G-UOH and G-C-UOH for Galleria mellonella larvae and Y-C-G-AOH, Y-G-U-AOH and G-Y-U-GOH for Drosophila melanogaster flies. Thus the introduction of the central scission in insect 26S rRNA results in the generation of a unique 3′-terminus and does not arise from random cleavage of the polynucleotide chain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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