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. 1974 Sep;141(3):647–653. doi: 10.1042/bj1410647

Studies on the fractionation of total transfer ribonucleic acid and purification of an alanine transfer ribonucleic acid from chick embryo

M P Jacob Spurgeon Anandaraj 1, Joseph D Cherayil 1
PMCID: PMC1168169  PMID: 4463955

Abstract

Chick embryo tRNA, prepared by a simple large-scale method, was fractionated on three different ion-exchange columns. In all cases simple chromatographic patterns for various tRNA species were observed, indicating the presence of only a few major species of tRNA for each amino acid. By repeated chromatography one species of alanine tRNA was purified to approx. 80% purity. T1 ribonuclease digest of this purified tRNA gave a simple chromatographic pattern. Because of the simplicity of the method of preparation of tRNA from this readily available source and the presence of only a few species of tRNA for each amino acid, chick embryo is suited for the study of tRNA and its various functions in higher systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anandaraj M. P., Cherayil J. D. A simple method for large-scale preparation of chick embryo tRNA. Anal Biochem. 1974 Mar;58(1):190–194. doi: 10.1016/0003-2697(74)90457-6. [DOI] [PubMed] [Google Scholar]
  2. BRUNNGRABER E. F. A simplified procedure for the preparation of "soluble" RNA from rat liver. Biochem Biophys Res Commun. 1962 Jun 19;8:1–3. doi: 10.1016/0006-291x(62)90223-1. [DOI] [PubMed] [Google Scholar]
  3. Cherayil J. D., Bock R. M. A column chromatographic procedure for the fractionation of s-RNA. Biochemistry. 1965 Jun;4(6):1174–1183. doi: 10.1021/bi00882a028. [DOI] [PubMed] [Google Scholar]
  4. Dudock B. S., Katz G., Taylor E. K., Holley R. W. Primary structure of wheat germ phenylalanine transfer RNA. Proc Natl Acad Sci U S A. 1969 Mar;62(3):941–945. doi: 10.1073/pnas.62.3.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
  6. Holland J. J., Taylor M. W., Buck C. A. Chromatographic differences between tyrosyl transfer RNA from different mammalian cells. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2437–2444. doi: 10.1073/pnas.58.6.2437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaneko I., Doi R. H. Alteration of valyl-sRNA during sporulation of bacillus subtilis. Proc Natl Acad Sci U S A. 1966 Mar;55(3):564–571. doi: 10.1073/pnas.55.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keith G., Picaud F., Weissenbach J., Ebel J. P., Petrissant G., Dirheimer G. The primary structure of rabbit liver tRNA Phe and its comparison with known tRNA Phe sequences. FEBS Lett. 1973 May 1;31(3):345–347. doi: 10.1016/0014-5793(73)80138-3. [DOI] [PubMed] [Google Scholar]
  9. Pearson R. L., Hancher C. W., Weiss J. F., Holladay D. W., Kelmers A. D. Preparation of crude transfer RNA and chromatographic purification of five transfer RNAs from calf liver. Biochim Biophys Acta. 1973 Jan 19;294(2):236–249. [PubMed] [Google Scholar]
  10. Reeves R. H., Imura N., Schwam H., Weiss G. B., Schulman L. H., Chambers R. W. Transfer RNA, I. Isolation and characterization of a new yeast alanine transfer RNA. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1450–1457. doi: 10.1073/pnas.60.4.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SUEOKA N., KANO-SUEOKA T. A SPECIFIC MODIFICATION OF LEUCYL-SRNA OF ESCHERICHIA COLI AFTER PHAGE T2 INFECTION. Proc Natl Acad Sci U S A. 1964 Dec;52:1535–1540. doi: 10.1073/pnas.52.6.1535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  13. Smith C. J., Smith P. M., Herbert E. Isolation of amino acyl oligonucleotides from T-1 ribonuclease digests of soluble ribonucleic acid charged with serine, glycine, threonine, and alanine. Biochemistry. 1966 Apr;5(4):1323–1332. doi: 10.1021/bi00868a028. [DOI] [PubMed] [Google Scholar]
  14. Staehelin M., Rogg H., Baguley B. C., Ginsberg T., Wehrli W. Structure of a mammalian serine tRNA. Nature. 1968 Sep 28;219(5161):1363–1365. doi: 10.1038/2191363a0. [DOI] [PubMed] [Google Scholar]
  15. Taylor M. W., Buck C. A., Granger G. A., Holland J. J. Chromatographic alterations in transfer RNA's accompanying speciation, differentiation and tumor formation. J Mol Biol. 1968 May 14;33(3):809–828. doi: 10.1016/0022-2836(68)90321-5. [DOI] [PubMed] [Google Scholar]
  16. White B. N., Tener G. M. Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus. J Mol Biol. 1973 Mar 15;74(4):635–651. doi: 10.1016/0022-2836(73)90054-5. [DOI] [PubMed] [Google Scholar]
  17. Yang S. S., Comb D. G. Distribution of multiple forms of lysyl transfer RNA during early embryogenesis of sea urchin, Lytechinus variegatus. J Mol Biol. 1968 Jan 14;31(1):138–142. doi: 10.1016/0022-2836(68)90062-4. [DOI] [PubMed] [Google Scholar]

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