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. 1974 Sep;141(3):675–682. doi: 10.1042/bj1410675

Calcium and thiol reactivity of human plasma clotting factor XIII

Rodney D Cooke 1,*, Timothy C Pestell 1,†, J John Holbrook 1
PMCID: PMC1168172  PMID: 4463957

Abstract

1. The reaction of iodoacetate, 2-chloromercuri-4-nitrophenol and 5,5′-dithiobis-(2-nitrobenzoate) with thrombin-cleaved Factor XIII (i.e. Factor XIIIa) was accompanied by enzyme inhibition. 2. The reaction with iodoacetate and 5,5′-dithiobis-(2-nitrobenzoate) was absolutely dependent on Ca2+, and the rate of reaction increased with the Ca2+ concentration up to very high, non-physiological concentrations. 3. 2-Chloromercuri-4-nitrophenol reacted with Factor XIIIa in the absence of Ca2+, but at a much slower rate. 4. Stopped-flow methods were used to quantify the reaction with 5,5′-dithiobis-(2-nitro-benzoate) because of the Ca2+-dependent dissociation of Factor XIIIa (a′2b2) and subsequent aggregation of the a′ chains into turbid precipitates. 5. The 3-carboxy-4-nitrothio-phenolate released was consistent with the reaction of 2 thiol groups/molecule of Factor XIIIa. The isolated b chains of Factor XIII did not react with either of the chromophoric reagents. This indicated that the a′ chains of Factor XIIIa were responsible for the thiol reactivity of the enzyme. 6. The Ca2+ dependence of the enzyme inhibition by these thiol reagents was very dependent on protein concentration. This is discussed in relation to the Ca2+-induced dissociation of Factor XIIIa. 7. The acceptor substrate, casein, decreased the Ca2+ concentration required for enzyme inhibition by both the mercurial and the aromatic disulphide compounds. Dansylcadaverine did not affect Ca2+ dependence of inhibition.

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Selected References

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