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. 1974 Sep;141(3):909–911. doi: 10.1042/bj1410909

A set of surface proteins common to the circulating human platelet and lymphocyte (Short Communication)

M J A Tanner 1,2, D H Boxer 1,2, Jane Cumming 1,2, J Verrier-Jones 1,2
PMCID: PMC1168196  PMID: 4463970

Abstract

The surface proteins of the circulating human platelet and lymphocyte were labelled by using the lactoperoxidase iodination method. Polyacrylamide-gel electrophoresis showed that four corresponding labelled proteins are found on the surface of each cell type. The most intensely labelled protein contains little or no carbohydrate, but the remaining labelled proteins are all glycoproteins. The major labelled band from each cell was isolated and comparative peptide `maps' showed that the two proteins are closely similar. The surface proteins of the lymphocyte and platelet are distinct from those on the erythrocyte, the remaining major type of circulating cell.

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Selected References

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  1. Allan D., Crumpton M. J. Solubilization of pig lymphocyte plasma membrane and fractionation of some of the components. Biochem J. 1971 Aug;123(5):967–975. doi: 10.1042/bj1230967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barber A. J., Jamieson G. A. Isolation and characterization of plasma membranes from human blood platelets. J Biol Chem. 1970 Dec 10;245(23):6357–6365. [PubMed] [Google Scholar]
  3. Barber A. J., Jamieson G. A. Isolation of glycopeptides from low- and high-density platelet plasma membranes. Biochemistry. 1971 Dec 7;10(25):4711–4717. doi: 10.1021/bi00801a018. [DOI] [PubMed] [Google Scholar]
  4. Boxer D. H., Jenkins R. E., Tanner M. J. The organization of the major protein of the human erythrocyte membrane. Biochem J. 1974 Mar;137(3):531–534. doi: 10.1042/bj1370531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bretscher M. S. Major human erythrocyte glycoprotein spans the cell membrane. Nat New Biol. 1971 Jun 23;231(25):229–232. doi: 10.1038/newbio231229a0. [DOI] [PubMed] [Google Scholar]
  6. Harris R., Ukaejiofo E. O. Tissue typing using a routine one-step lymphocyte separation procedure. Br J Haematol. 1970 Feb;18(2):229–235. doi: 10.1111/j.1365-2141.1970.tb01436.x. [DOI] [PubMed] [Google Scholar]
  7. Hubbard A. L., Cohn Z. A. The enzymatic iodination of the red cell membrane. J Cell Biol. 1972 Nov;55(2):390–405. doi: 10.1083/jcb.55.2.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jondal M., Klein G. Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. J Exp Med. 1973 Dec 1;138(6):1365–1378. doi: 10.1084/jem.138.6.1365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klinman N. R., Taylor R. B. General methods for the study of cells and serum during the immune response: the response to dinitrophenyl in mice. Clin Exp Immunol. 1969 Apr;4(4):473–487. [PMC free article] [PubMed] [Google Scholar]
  10. Nachman R. L., Ferris B. Studies on the proteins of human platelet membranes. J Biol Chem. 1972 Jul 25;247(14):4468–4475. [PubMed] [Google Scholar]
  11. Phillips D. R. Effect of trypsin on the exposed polypeptides and glycoproteins in the human platelet membrane. Biochemistry. 1972 Nov 21;11(24):4582–4588. doi: 10.1021/bi00774a025. [DOI] [PubMed] [Google Scholar]
  12. Rabellino E., Colon S., Grey H. M., Unanue E. R. Immunoglobulins on the surface of lymphocytes. I. Distribution and quantitation. J Exp Med. 1971 Jan 1;133(1):156–167. doi: 10.1084/jem.133.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vitetta E. S., Baur S., Uhr J. W. Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes. J Exp Med. 1971 Jul 1;134(1):242–264. doi: 10.1084/jem.134.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969 Jul;30(1):148–152. doi: 10.1016/0003-2697(69)90383-2. [DOI] [PubMed] [Google Scholar]
  15. Zimmerman T. S., Müller-Eberhard H. J. Complement-induced platelet protein alterations. Science. 1973 Jun 15;180(4091):1183–1185. doi: 10.1126/science.180.4091.1183. [DOI] [PubMed] [Google Scholar]

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