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. 1974 Sep;142(3):605–609. doi: 10.1042/bj1420605

Protein kinase activity stimulated by adenosine 3′:5′-cyclic monophosphate in synaptic-membrane fragments from ox brain. Inhibition of intrinsic activity by free and membrane-bound calcium ions

Malcolm Weller 1,*, Richard Rodnight 1,†
PMCID: PMC1168325  PMID: 4377212

Abstract

1. Cyclic AMP-stimulated protein kinase activity phosphorylating intrinsic substrates in preparations of synaptic-membrane fragments from ox cerebral cortex was examined in relation to (a) the content of membrane-bound Ca2+ in the preparations and (b) added Ca2+ in the assay medium. 2. Centrifugal washing of synaptic-membrane fragments with buffered ethane dioxybis(ethylamine)tetra-acetate solutions decreased bound Ca2+ from 2.8±0.4 (s.d.) to 0.9±0.3nmol/mg of protein. In washed preparations basal protein kinase activity was increased by about 40% and the cyclic AMP-stimulated activity by about 15%. Addition of Ca2+ in the concentration range 5–50μm to the assay medium progressively inhibited the kinase activity of the washed preparations; in this range of Ca2+ concentration the basal activity was inhibited more than the stimulated activity. 3. In unwashed preparations concentrations of Ca2+ above 100μm inhibited the cyclic AMP-stimulated activity more than the basal activity. 4. The inhibitory effect of several concentrations of Ca2+ was examined in relation to cyclic AMP concentration; no evidence for competition between Ca2+ and cyclic AMP for a site on the enzyme was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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