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. 1974 Sep;142(3):653–658. doi: 10.1042/bj1420653

The effect of starvation on phosphodiesterase activity and the content of adenosine 3′:5′-cyclic monophosphate in isolated mouse pancreatic islets

Kirsten Capito 1, Carl Jørgen Hedeskov 1
PMCID: PMC1168331  PMID: 4377213

Abstract

1. The concentration of cyclic AMP and the activity of phosphodiesterase were measured in isolated pancreatic islets from fed or 48h-starved mice. 2. Two different phosphodiesterases were detected. Neither the maximum activity nor the Km values of these enzymes were changed by starvation. 3. The concentration of cyclic AMP in non-incubated islets was the same in islets from fed and starved mice. 4. Incubation with 3.3mm-glucose for 5–30min had no effect on the concentration of cyclic AMP, irrespective of the nutritional state of the mice. Incubation with 16.7mm-glucose for 5–30min raised the concentration of cyclic AMP by about 30% in islets from fed mice. This rise was prevented by addition of mannoheptulose (3mg/ml). Incubation with 16.7mm-glucose had no effect on the cyclic AMP content in islets from starved mice. 5. In islets from fed mice 10min incubation with 5mm-caffeine had no effect on the concentration of cyclic AMP in the presence of 3.3 or 16.7mm-glucose, whereas the cyclic AMP content was increased approx. 150% in islets from starved mice. 6. After 10min incubation with 1mm-3-isobutyl-1-methylxanthine in the presence of 3.3 or 16.7mm-glucose the concentration of cyclic AMP was raised by 250% in islets from fed mice and by 400% in islets from starved mice. 7. A threefold function of glucose in the insulin-secretory process is suggested, according to which the decreased islet glucose metabolism is the primary defect in the insulin-secretory mechanism during starvation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashcroft S. J.H., Randle P. J., Täljedal I. -B. Cyclic nucleotide phosphodiesterase activity in normal mouse pancreatic islets. FEBS Lett. 1972 Feb 15;20(3):263–266. doi: 10.1016/0014-5793(72)80082-6. [DOI] [PubMed] [Google Scholar]
  2. Ashcroft S. J., Hedeskov C. J., Randle P. J. Glucose metabolism in mouse pancreatic islets. Biochem J. 1970 Jun;118(1):143–154. doi: 10.1042/bj1180143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brisson G. R., Malaisse-Lagae F., Malaisse W. J. The stimulus-secretion coupling of glucose-induced insulin release. VII. A proposed site of action for adenosine-3',5'-cyclic monophosphate. J Clin Invest. 1972 Feb;51(2):232–241. doi: 10.1172/JCI106808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cerasi E., Luft R. Diabetes mellitus--a disorder of cellular information transmission? Horm Metab Res. 1970 Jul;2(4):246–249. doi: 10.1055/s-0028-1095082. [DOI] [PubMed] [Google Scholar]
  5. Charles M. A., Fanska R., Schmid F. G., Forsham P. H., Grodsky G. M. Adenosine 3',5'-monophosphate in pancreatic islets: glucose-induced insulin release. Science. 1973 Feb 9;179(4073):569–571. doi: 10.1126/science.179.4073.569. [DOI] [PubMed] [Google Scholar]
  6. Coll-Garcia E., Gill J. R. Insulin release by isolated pancreatic islets of the mouse incubated in vitro. Diabetologia. 1969 Apr;5(2):61–66. doi: 10.1007/BF01211999. [DOI] [PubMed] [Google Scholar]
  7. Cooper R. H., Ashcroft S. J., Randle P. J. Concentration of adenosine 3':5'-cyclic monophosphate in mouse pancreatic islets measured by a protein-binding radioassay. Biochem J. 1973 Jun;134(2):599–605. doi: 10.1042/bj1340599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cooper R. H., McPherson M., Schofield J. G. The effect of prostaglandins on ox pituitary content of adenosine 3':5'-cyclic monophosphate and the release of growth hormone. Biochem J. 1972 Mar;127(1):143–154. doi: 10.1042/bj1270143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Davis B., Lazarus N. R. Insulin release from mouse islets. Effect of glucose and hormones on adenylate cyclase. Biochem J. 1972 Sep;129(2):373–379. doi: 10.1042/bj1290373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grey N. J., Goldring S., Kipnis D. M. The effect of fasting, diet, and actinomycin D on insulin secretion in the rat. J Clin Invest. 1970 May;49(5):881–889. doi: 10.1172/JCI106307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grill V., Cerasi E. Activation by glucose of adenyl cyclase in pancreatic islets of the rat. FEBS Lett. 1973 Jul 15;33(3):311–314. doi: 10.1016/0014-5793(73)80218-2. [DOI] [PubMed] [Google Scholar]
  13. Hedeskov C. J., Capito K. The effect of starvation on insulin secretion and glucose metabolism in mouse pancreatic islets. Biochem J. 1974 Jun;140(3):423–433. doi: 10.1042/bj1400423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Howell S. L., Green I. C., Montague W. A possible role of adenylate cyclase in the long-tern dietary regulation of insulin secretion from rat islets of Langerhans. Biochem J. 1973 Oct;136(2):343–349. doi: 10.1042/bj1360343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Howell S. L., Montague W. Adenylate cyclase activity in isolated rat islets of Langerhans. Effects of agents which alter rates of insulin secretion. Biochim Biophys Acta. 1973 Aug 17;320(1):44–52. doi: 10.1016/0304-4165(73)90163-3. [DOI] [PubMed] [Google Scholar]
  16. KISSANE J. M., ROBINS E. The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem. 1958 Jul;233(1):184–188. [PubMed] [Google Scholar]
  17. Kuo W. N., Hodgins D. S., Kuo J. F. Adenylate cyclase in islets of Langerhans. Isolation of islets and regulation of adenylate cyclase activity by various hormones and agents. J Biol Chem. 1973 Apr 25;248(8):2705–2711. [PubMed] [Google Scholar]
  18. Malaisse W. J., Malaisse-Lagae F., Mayhew D. A possible role for the adenylcyclase system in insulin secretion. J Clin Invest. 1967 Nov;46(11):1724–1734. doi: 10.1172/JCI105663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Malaisse W. J., Malaisse-Lagae F., Wright P. H. Effect of fasting upon insulin secretion in the rat. Am J Physiol. 1967 Oct;213(4):843–848. doi: 10.1152/ajplegacy.1967.213.4.843. [DOI] [PubMed] [Google Scholar]
  20. Montague W., Cook J. R. The role of adenosine 3':5'-cyclic monophosphate in the regulation of insulin release by isolated rat islets of Langerhans. Biochem J. 1971 Mar;122(1):115–120. doi: 10.1042/bj1220115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Montague W., Howell S. L. The mode of action of adenosine 3':5'-cyclic monophosphate in mammalian islets of Langerhans. Preparation and properties of islet-cell protein phosphokinase. Biochem J. 1972 Sep;129(3):551–560. doi: 10.1042/bj1290551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sams D. J., Montague W. The role of adenosine 3':5'-cyclic monophosphate in the regulation of insulin release. Properties of islet-cell adenosine 3':5'-cyclic monophosphate phosphodiesterase. Biochem J. 1972 Oct;129(4):945–952. doi: 10.1042/bj1290945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Selawry H., Gutman R., Fink G., Recant L. The effect of starvation on tissue adenosine 3'-5' monophosphate levels. Biochem Biophys Res Commun. 1973 Mar 5;51(1):198–204. doi: 10.1016/0006-291x(73)90528-7. [DOI] [PubMed] [Google Scholar]
  24. Voyles N., Gutman R. A., Selawry H., Fink G., Penhos J. C., Recant L. Interaction of various stimulators and inhibitors on insulin secretion in vitro. Horm Res. 1973;4(2):65–73. doi: 10.1159/000178291. [DOI] [PubMed] [Google Scholar]

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