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. 1974 Sep;142(3):667–671. doi: 10.1042/bj1420667

Heterogeneous distribution of enzymes among plasma-membrane fragments sedimenting with the microsomal fraction of rat liver

Kenneth A Norris 1, Miloslav Dobrota 1, Faiz S Issa 1, Richard H Hinton 1, Eric Reid 1
PMCID: PMC1168333  PMID: 4377214

Abstract

Plasma-membrane fragments recovered in the microsomal fraction of rat liver homogenates were shown to be heterogeneous in density. It was demonstrated that 5′-nucleotidase, the most commonly used plasma-membrane marker, is concentrated in the lightest subfraction. Two of the published procedures for the isolation of plasma-membrane fragments from the microsomal fraction (Touster et al., 1970; Hinton et al., 1971) are shown to give products which are not representative of all the plasma-membrane fragments of microsomal size, and it is argued that a third procedure (House & Weidemann, 1970) is likely to give a similar product.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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