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. 1974 Sep;142(3):691–693. doi: 10.1042/bj1420691

Stimulation by 6-N,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate and glucocorticoids of phosphoenolpyruvate carboxykinase in the isolated perfused rat liver (Short Communication)

Wieland B Huttner 1, Wilhelm Krone 1, Hans J Seitz 1, Wolfgang Tarnowski 1
PMCID: PMC1168336  PMID: 4377216

Abstract

Dibutyryl cyclic AMP stimulated the activity of phosphoenolpyruvate carboxykinase in perfused livers of rats, fed on a low-protein diet, linearly over a 6h period. The enzyme activity was also significantly elevated by dexamethasone, the effect being considerably lower than that of the cyclic nucleotide. Since the time-course of phosphoenolpyruvate carboxykinase activity in response to dibutyryl cyclic AMP resembled that observed after dibutyryl cyclic AMP injection into intact animals, it is suggested that induction of the enzyme in vivo is due to a direct action of the cyclic nucleotide on the liver. Combined administration of dibutyryl cyclic AMP and glucocorticoids did not lead to an additive increase of liver phosphoenolpyruvate carboxykinase activity, either in vivo or in the perfused organ.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnett C. A., Wicks W. D. Regulation of phosphoenolpyruvate carboxykinase and tyrosine transaminase in hepatoma cell cultures. I. Effects of glucocorticoids, N 6 ,O 2' -dibutyryl cyclic adenosine 3',5'-monophosphate and insulin in Reuber H35 cells. J Biol Chem. 1971 Dec 10;246(23):7201–7206. [PubMed] [Google Scholar]
  2. Exton J. H. Gluconeogenesis. Metabolism. 1972 Oct;21(10):945–990. doi: 10.1016/0026-0495(72)90028-5. [DOI] [PubMed] [Google Scholar]
  3. Foster D. O., Ray P. D., Lardy H. A. Studies on the mechanisms underlying adaptive changes in rat liver phosphoenolpyruvate carboxykinase. Biochemistry. 1966 Feb;5(2):555–562. doi: 10.1021/bi00866a022. [DOI] [PubMed] [Google Scholar]
  4. Menahan L. A., Wieland O. The role of thyroid function in the metabolism of perfused rat liver with particular reference to gluconeogenesis. Eur J Biochem. 1969 Aug;10(1):188–194. doi: 10.1111/j.1432-1033.1969.tb00672.x. [DOI] [PubMed] [Google Scholar]
  5. SCHIMASSEK H. [Metabolites of carbohydrate metabolism in the isolated perfused rat liver]. Biochem Z. 1963;336:460–467. [PubMed] [Google Scholar]
  6. Seubert W., Huth W. On the mechanism of gluconeogenesis and its regulation. II. The mechanism of gluconeogenesis from pyruvate and fumarate. Biochem Z. 1965 Nov 15;343(2):176–191. [PubMed] [Google Scholar]
  7. Wicks W. D. Induction of hepatic enzymes by adenosine 3',5'-monophosphate in organ culture. J Biol Chem. 1969 Jul 25;244(14):3941–3950. [PubMed] [Google Scholar]
  8. Wicks W. D., Kenney F. T., Lee K. L. Induction of hepatic enzyme synthesis in vivo by adenosine 3', 5'-monophosphate. J Biol Chem. 1969 Nov 10;244(21):6008–6013. [PubMed] [Google Scholar]
  9. Wicks W. D., Lewis W., McKibbin J. B. Induction of phosphoenolpyruvate carboxykinase by N 6 , O 2 '-dibutyryl cyclic AMP in rat liver. Biochim Biophys Acta. 1972 Mar 30;264(1):177–185. doi: 10.1016/0304-4165(72)90129-8. [DOI] [PubMed] [Google Scholar]

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