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. 1974 Nov;143(2):491–493. doi: 10.1042/bj1430491

Proteolysis of rabbit immunoglobulin M by papain (Short Communication)

Barbara M McIlroy 1, George T Stevenson 1
PMCID: PMC1168411  PMID: 4218955

Abstract

1. Digestion of rabbit immunoglobulin M (IgM) by papain for 5h, in the absence of reducing agent, gives Fabμ and Fc5μ fragments in high yield. Shorter periods give fragment Fc5μ with one or more Fabμ fragments still attached. 2. Reducing agent in the absence of a denaturant cleaves rabbit IgM into half-subunits, each containing one μ chain and one light chain. Digestion by papain in the presence of such a reducing agent destroys the Fcμ domains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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