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. 1974 Nov;144(2):311–318. doi: 10.1042/bj1440311

Transport of catecholamines by resealed chromaffin-granule `ghosts'

John H Phillips 1,*
PMCID: PMC1168498  PMID: 4477958

Abstract

A method is described for the preparation of resealed chromaffin-granule `ghosts'. The lysis and rapid purification procedures provide `ghosts' in approximately 70% yield from crude granules; the preparation contains 0.1μmol of catecholamine/mg of protein (as compared with 2.8μmol/mg in unlysed granules), of which about one third is inside the `ghosts'. The `ghosts' retain their ability to accumulate catecholamines, a process dependent on Mg-ATP and inhibited by reserpine, and a simple assay for this transport is described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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