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. 1997 Aug 1;16(15):4560–4567. doi: 10.1093/emboj/16.15.4560

Reconstitution of the protein insertion machinery of the mitochondrial inner membrane.

V Haucke 1, G Schatz 1
PMCID: PMC1170082  PMID: 9303300

Abstract

We have reconstituted the protein insertion machinery of the yeast mitochondrial inner membrane into proteoliposomes. The reconstituted proteoliposomes have a distinct morphology and protein composition and correctly insert the ADP/ATP carrier (AAC) and Tim23p, two multi-spanning integral proteins of the mitochondrial inner membrane. The reconstituted system requires a membrane potential, but not Tim44p or mhsp70, both of which are required for the ATP-driven translocation of proteins into the matrix. The protein insertion machinery can thus operate independently of the energy-transducing Tim44p-mhsp70 complex.

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Selected References

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